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Tag Archives: Illumina
DSBCapture: in situ capture and sequencing of DNA breaks
August 2016 Authors: Stefanie V Lensing, Giovanni Marsico, Robert Hänsel-Hertsch, Enid Y Lam, David Tannahill & Shankar Balasubramanian Info: Scientists at Cancer Research UK developed and validated a new sequencing-based protocol to spot double-stranded DNA breaks in situ; these regions … more »
Posted in Citation
Tagged BluePippin, DNA Sequencing, Illumina, Methods, NextSeq
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Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle
August 2016 Authors: Eduardo Casas, Guohong Cai, Larry A. Kuehn, Karen B. Register, Tara G. McDaneld, John D. Neill Info: USDA scientists performed a longitudinal study of young cows to identify microRNAs linked to a serum antibody produced after exposure … more »
Posted in Citation
Tagged HiSeq, Illumina, microRNA, Pippin Prep, RNA sequencing
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Comparative genomics and transcriptomics of Pichia pastoris
August 2016 Authors: Kerry R. Love, Kartik A. Shah, Charles A. Whittaker, Jie Wu, M. Catherine Bartlett, Duanduan Ma, Rachel L. Leeson, Margaret Priest, Jonathan Borowsky, Sarah K. Young and J. Christopher Love Info: To provide a foundation for engineering … more »
Posted in Citation
Tagged DNA Sequencing, HiSeq, Illumina, Pippin Prep
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RADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data
August 2016 Authors: Hoffberg SL, Kieran TJ, Catchen JM, Devault A, Faircloth BC, Mauricio R, Glenn TC Info: In this methods paper, scientists combine double-digest RAD-seq with capture technology to reduce costs and increase efficiency. The protocol, which was validated … more »
Posted in Citation
Tagged ddRADseq, genotyping, Illumina, Methods, NextSeq, Pippin Prep, Target Enrichment
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Circulating Extracellular RNA Markers of Liver Regeneration
July 2016 Authors: Irene K. Yan, Xue Wang, Yan W. Asmann, Hiroaki Haga, Tushar Patel Info: Mayo Clinic researchers performed partial hepatectomies in mice and then monitored RNA activity in serum samples to find a signature associated with liver regeneration. … more »
Posted in Citation
Tagged cfRNA, Illumina, MiSeq, Pippin Prep, RNA sequencing
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Genome-wide identification of enhancer elements
June 2016 Authors: Sarah Tulin, Julius C. Barsi, Carlo Bocconcelli, and Joel Smith Info: The authors (from Woods Hole and Cal Tech) present a genome-wide regulatory element database for the sea urchin embryo. To do this, they developed a new … more »
PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer
June 2016 Authors: Jennifer M. Spangle, Koen M. Dreijerink, Anna C. Groner, Hailing Cheng, Carolynn E. Ohlson, Jaime Reyes, Charles Y. Lin, James Bradner, Jean J. Zhao, Thomas M. Roberts, and Myles Brown Info: In this breast cancer research study, … more »
Posted in Citation
Tagged ChIP-Seq, Illumina, Pippin Prep
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PELE-seq Increases SNP Calling Accuracy by Reducing Sequencing Errors
Scientists at the University of Oregon have published a new method to detect PCR and sequencing errors that should help other researchers track rare SNPs with greater accuracy. PELE-seq, which gets our vote for best new protocol name, can be … more »
Posted in Blog
Tagged ddRADseq, genotyping, HiSeq, Illumina, Methods, Pippin Prep
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Double Pippin for Optimized RAD-seq
Scientists in China and the UK recently published an open-access optimized protocol for RAD-seq in the Theoretical and Applied Genetics journal. The method is targeted at large studies of plants and enables users to specify sequence coverage parameters. From lead … more »
Urban Mice: NYC Study Sheds Light on Population Diversity and Changes
They may make subway riders shudder, but New York City mice are the stars of a cool new paper outlining their evolutionary history during a rapid period of urbanization. Scientists from the City University of New York used ddRAD-seq to … more »
Posted in Blog
Tagged ddRADseq, genotyping, HiSeq, Illumina, Pippin Prep
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