-
Recent Posts
- Leveraging the power of long reads for targeted sequencing
- Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints
- CTLR-Seq Protocol
- Processing frozen archival human DNA samples for large-scale SQK-LSK114 Oxford Nanopore long-read DNA sequencing SOP v1
- Identifying the best PCR enzyme for library amplification in NGS
Tags
10x Genomics ABRF agbt Amplicon ASHG ASMS BluePippin ChIP-Seq ddRADseq DNA Sequencing Featured genotyping High Pass HiSeq HLA HLS HLS-CATCH Illumina ILMN Ion Torrent isoseq Mate-pair Sequencing Methods microRNA miRNA miRNA isolation MiSeq ONT oxford nanopore PacBio PAG Paired-end Sequencing PippinHT Pippin Prep Pippin System podcast Protein Mass Spec Protein Size Fractionation proteomics RAD-seq RNA-seq RNA sequencing SageELF SageHLS Tips and ApplicationsRecent Comments
- Alex on And Now, a Word from Our Customer Service Department
- Donna on And Now, a Word from Our Customer Service Department
- Tony Gill on And Now, a Word from Our Customer Service Department
- Alex on And Now, a Word from Our Customer Service Department
- Andrea on And Now, a Word from Our Customer Service Department
Archives
- December 2024
- July 2024
- June 2024
- April 2024
- February 2024
- January 2024
- December 2023
- August 2023
- June 2023
- March 2023
- February 2023
- January 2023
- November 2022
- September 2022
- April 2022
- January 2022
- September 2021
- July 2021
- June 2021
- May 2021
- February 2021
- January 2021
- November 2020
- October 2020
- July 2020
- May 2020
- April 2020
- March 2020
- February 2020
- January 2020
- November 2019
- August 2019
- July 2019
- June 2019
- May 2019
- April 2019
- March 2019
- February 2019
- January 2019
- December 2018
- October 2018
- September 2018
- May 2018
- April 2018
- March 2018
- February 2018
- January 2018
- December 2017
- November 2017
- October 2017
- September 2017
- August 2017
- July 2017
- June 2017
- May 2017
- April 2017
- March 2017
- February 2017
- January 2017
- December 2016
- November 2016
- October 2016
- September 2016
- August 2016
- July 2016
- June 2016
- May 2016
- April 2016
- March 2016
- February 2016
- January 2016
- December 2015
- November 2015
- October 2015
- September 2015
- August 2015
- July 2015
- June 2015
- May 2015
- April 2015
- March 2015
- February 2015
- January 2015
- December 2014
- November 2014
- October 2014
- September 2014
- August 2014
- July 2014
- June 2014
- May 2014
- April 2014
- March 2014
- February 2014
- January 2014
- December 2013
- November 2013
- October 2013
- September 2013
- August 2013
- July 2013
- June 2013
- May 2013
- April 2013
- March 2013
- February 2013
- January 2013
- December 2012
- November 2012
- October 2012
- September 2012
- August 2012
- July 2012
- June 2012
- May 2012
- April 2012
- March 2012
- February 2012
- December 2011
- October 2011
- September 2011
- June 2011
- February 2011
- November 2010
- October 2010
- August 2010
Tag Archives: Illumina
DSBCapture: in situ capture and sequencing of DNA breaks
August 2016 Authors: Stefanie V Lensing, Giovanni Marsico, Robert Hänsel-Hertsch, Enid Y Lam, David Tannahill & Shankar Balasubramanian Info: Scientists at Cancer Research UK developed and validated a new sequencing-based protocol to spot double-stranded DNA breaks in situ; these regions … more »
Posted in Citation
Tagged BluePippin, DNA Sequencing, Illumina, Methods, NextSeq
Comments Off on DSBCapture: in situ capture and sequencing of DNA breaks
Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle
August 2016 Authors: Eduardo Casas, Guohong Cai, Larry A. Kuehn, Karen B. Register, Tara G. McDaneld, John D. Neill Info: USDA scientists performed a longitudinal study of young cows to identify microRNAs linked to a serum antibody produced after exposure … more »
Posted in Citation
Tagged HiSeq, Illumina, microRNA, Pippin Prep, RNA sequencing
Comments Off on Association of MicroRNAs with Antibody Response to Mycoplasma bovis in Beef Cattle
Comparative genomics and transcriptomics of Pichia pastoris
August 2016 Authors: Kerry R. Love, Kartik A. Shah, Charles A. Whittaker, Jie Wu, M. Catherine Bartlett, Duanduan Ma, Rachel L. Leeson, Margaret Priest, Jonathan Borowsky, Sarah K. Young and J. Christopher Love Info: To provide a foundation for engineering … more »
Posted in Citation
Tagged DNA Sequencing, HiSeq, Illumina, Pippin Prep
Comments Off on Comparative genomics and transcriptomics of Pichia pastoris
RADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data
August 2016 Authors: Hoffberg SL, Kieran TJ, Catchen JM, Devault A, Faircloth BC, Mauricio R, Glenn TC Info: In this methods paper, scientists combine double-digest RAD-seq with capture technology to reduce costs and increase efficiency. The protocol, which was validated … more »
Posted in Citation
Tagged ddRADseq, genotyping, Illumina, Methods, NextSeq, Pippin Prep, Target Enrichment
Comments Off on RADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data
Circulating Extracellular RNA Markers of Liver Regeneration
July 2016 Authors: Irene K. Yan, Xue Wang, Yan W. Asmann, Hiroaki Haga, Tushar Patel Info: Mayo Clinic researchers performed partial hepatectomies in mice and then monitored RNA activity in serum samples to find a signature associated with liver regeneration. … more »
Posted in Citation
Tagged cfRNA, Illumina, MiSeq, Pippin Prep, RNA sequencing
Comments Off on Circulating Extracellular RNA Markers of Liver Regeneration
Genome-wide identification of enhancer elements
June 2016 Authors: Sarah Tulin, Julius C. Barsi, Carlo Bocconcelli, and Joel Smith Info: The authors (from Woods Hole and Cal Tech) present a genome-wide regulatory element database for the sea urchin embryo. To do this, they developed a new … more »
PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer
June 2016 Authors: Jennifer M. Spangle, Koen M. Dreijerink, Anna C. Groner, Hailing Cheng, Carolynn E. Ohlson, Jaime Reyes, Charles Y. Lin, James Bradner, Jean J. Zhao, Thomas M. Roberts, and Myles Brown Info: In this breast cancer research study, … more »
Posted in Citation
Tagged ChIP-Seq, Illumina, Pippin Prep
Comments Off on PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer
PELE-seq Increases SNP Calling Accuracy by Reducing Sequencing Errors
Scientists at the University of Oregon have published a new method to detect PCR and sequencing errors that should help other researchers track rare SNPs with greater accuracy. PELE-seq, which gets our vote for best new protocol name, can be … more »
Posted in Blog
Tagged ddRADseq, genotyping, HiSeq, Illumina, Methods, Pippin Prep
Comments Off on PELE-seq Increases SNP Calling Accuracy by Reducing Sequencing Errors
Double Pippin for Optimized RAD-seq
Scientists in China and the UK recently published an open-access optimized protocol for RAD-seq in the Theoretical and Applied Genetics journal. The method is targeted at large studies of plants and enables users to specify sequence coverage parameters. From lead … more »
Urban Mice: NYC Study Sheds Light on Population Diversity and Changes
They may make subway riders shudder, but New York City mice are the stars of a cool new paper outlining their evolutionary history during a rapid period of urbanization. Scientists from the City University of New York used ddRAD-seq to … more »
Posted in Blog
Tagged ddRADseq, genotyping, HiSeq, Illumina, Pippin Prep
Comments Off on Urban Mice: NYC Study Sheds Light on Population Diversity and Changes