Tag Archives: BluePippin

Complete Genome Sequence of a Klebsiella pneumoniae Strain Carrying blaNDM-1 on a Multidrug Resistance Plasmid

July/August 2016 Authors: Sean Conlan, Anna F. Lau, NISC Comparative Sequencing Program, Tara N. Palmore, Karen M. Frank, and Julia A. Segre Info: NIH scientists sequenced the genome of the first blaNDM-1-positive strain of Klebsiella pneumoniae detected in the institute’s hospital. They found three plasmids, … more »

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Analysis of tandem gene copies in maize chromosomal regions reconstructed from long sequence reads

July 2016 Authors: Jiaqiang Dong, Yaping Feng, Dibyendu Kumar, Wei Zhang, Tingting Zhu, Ming-Cheng Luo, and Joachim Messing Info: Scientists from Rutgers University and the University of California, Davis, analyzed copy number variation in the highly repetitive maize genome using … more »

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Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing

June 2016 Authors: Shruthi Sridhar Vembar, Matthew Seetin, Christine Lambert, Maria Nattestad, Michael C. Schatz, Primo Baybayan, Artur Scherf, and Melissa Laird Smith Info: Scientists in France and the US used PacBio sequencing to characterize the AT-rich genome of this … more »

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A survey of the sorghum transcriptome using single-molecule long reads

June 2016 Authors: Salah E. Abdel-Ghany, Michael Hamilton, Jennifer L. Jacobi, Peter Ngam, Nicholas Devitt, Faye Schilkey, Asa Ben-Hur & Anireddy S. N. Reddy Info: In this publication, scientists from Colorado State University and the National Center for Genome Resources … more »

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Sage Sizing Platforms Recommended for 10x Genomics Libraries

We’re pleased to report that 10x Genomics has released a new sample prep protocol for its Chromium platform that includes the BluePippin and PippinHT size selection platforms from Sage Science. 10x Genomics has gotten a lot of attention in recent … more »

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Scientists Use Multiple Technologies to Produce High-Quality Chinese Genome Assembly

A newly reported genome assembly of a Chinese individual, generated by scientists in China and the US, used long-read PacBio sequencing, short-read Illumina data, and BioNano Genomics physical maps to achieve remarkably high accuracy and contiguity. Along the way, the … more »

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Long-read sequencing and de novo assembly of a Chinese genome

June 2016 Authors: Lingling Shi, Yunfei Guo, Chengliang Dong, John Huddleston, Hui Yang, et al. Info: Scientists in China and the US analyzed the DNA and RNA of a Chinese individual, using PacBio sequencing and BioNano Genomics maps to produce … more »

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New GIAB Publication Characterizes Seven Genomes with 12 Technologies

The Genome in a Bottle Consortium is on a roll — and if you haven’t checked out the latest paper in Scientific Data, you’re missing out. “Extensive sequencing of seven human genomes to characterize benchmark reference materials” comes from lead … more »

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Extensive sequencing of seven human genomes to characterize benchmark reference materials

June 2016 Authors: Justin M. Zook, David Catoe, Jennifer McDaniel, Lindsay Vang, et al. Info: The Genome in a Bottle Consortium used 12 different DNA analysis technologies to generate deep data sets on seven genomes, five of which will become … more »

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NCSU Scientist Incorporates Genomics for Better Blueberry Resources

Hamid Ashrafi is working to breed higher-quality blueberries that are amenable to mechanical harvest, larger in size, tastier, and have a longer shelf life. As an assistant professor at North Carolina State University, Ashrafi is bringing genomic tools to a … more »

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