Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae

September 2014

Sean Conlan, Pamela J. Thomas, Clayton Deming, Morgan Park, Anna F. Lau, John P. Dekker, Evan S. Snitkin, Tyson A. Clark, Khai Luong, Yi Song, Yu-Chih Tsai, Matthew Boitano, Jyoti Dayal, Shelise Y. Brooks, Brian Schmidt, Alice C. Young, James W. Thomas, Gerard G. Bouffard, Robert W. Blakesley, NISC Comparative Sequencing Program, James C. Mullikin, Jonas Korlach, David K. Henderson, Karen M. Frank, Tara N. Palmore, Julia A. Segre

NIH scientists studied samples of antibiotic-resistant Enterobacteriaceae collected from patients and a hospital environment. They used the PacBio RS II DNA Sequencing System to analyze and assemble the bacterial genomes, and then traced transmission path based on genomic data. BluePippin was used to remove DNA fragments less than 5 Kb from the PacBio library.

Sci Transl Med 17 September 2014: Vol. 6, Issue 254

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