Minimum sample sizes for population genomics: an empirical study from an Amazonian plant species

February 2017

Alison G. Nazareno, Jordan B. Bemmels, Christopher W. Dick, Lúcia G. Lohmann

Scientists in Michigan and São Paulo, Brazil, performed ddRAD-seq on two separate populations of Amphirrhox longifolia to determine how many SNPs and organisms have to be included for a well-powered study. By resampling the data, they determined that only eight individuals are necessary for studies with at least 1,000 SNPs. The team used Pippin Prep to size fragments for sequencing.

Molecular Ecology Resources
DOI: 10.1111/1755-0998.12654

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