High-quality Arabidopsis thaliana Genome Assembly with Nanopore and HiFi Long Reads

September 2021

Authors:
Bo Wang, Xiaofei Yang, Yanyan Jia, Yu Xu, Peng Jia, Ningxin Dang, Songbo Wang, Tun Xu, Xixi Zhao, Shenghan Gao, Quanbin Dong, Kai Ye

Info:
The authors report on a high quality genome assembly of the model plant species Arabidopsis include two telomere-to-telomere assemblies (Chr 3 and 5). PacBio’s HiFi, Oxford Nanopore ultra-long reads and Hi-C (Illumina) were used.

BluePippin was used to size select large fragments from genomic extracts and for Hi-FI library construction. The SageHLS was used to collect DNA fragments >50kb for Oxford Nanopore ultra-long library prep (Genome Center of Grandomics [Wuhan, China]). .

Author Affiliations:
Xi’an Jiaotong University, Xi’an, China

Genomics, Proteomics & Bioinformatics
DOI: 10.1016/j.gpb.2021.08.003

This entry was posted in Citation and tagged , , , , . Bookmark the permalink.

Comments are closed.