Discovery of transgene insertion sites by high throughput sequencing of mate pair libraries

May 2014

Anuj Srivastava, Vivek M Philip, Ian Greenstein, Lucy B Rowe, Mary Barter, Cathleen Lutz and Laura G Reinholdt

Jackson Laboratory scientists developed a method to characterize and score transgene insertion sites in mice to help researchers more fully understand how these sites function and alter phenotypic outcomes. They used mate-pair sequencing with Illumina and performed size selection with a Pippin Prep.

BMC Genomics 2014, 15:367


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