Authors:
Emilie Cardona, Cervin Guyomar, Thomas Desvignes3, Jérôme Montfort , Samia Guendouz, John H. Postlethwait , Sandrine Skiba-Cassy, Julien Bobe
Info:
The researchers conducted a comprehensive study of circulating miRNAs (c-mRNAs) in biological fluids of the rainbow trout, with the aim of using c-miRNAs as biomarkers of reproductive and metabolic states of the fish species. The authors demonstrate strong fluid-specific miRNA signatures in plasma and ovarian fluids in particular. They identify potential biomarkers in response to high feeding rates in plasma (indicating active myogenesis) and biomarkers in response to ovulation ane egg quality in ovarian fluid.
PippinHT was used to isolate miRNA libraries (126-169 bp) from NEXTFlex small RNA kits (Perkin Elmer/Bioo Scientific).
Author Affiliations:
INRAE, LPGP, Fish Physiology and Genomics, Rennes, France
INRAE, Univ. Pau & Pays Adour, Saint-Pée-sur-Nivelle, France
Institute of Neurosciences, University of Oregon, Eugene OR
MGX, UMR, Institute of Functional Genomics, Montpellier, France
GenPhySE, University of Toulouse, INRAE, ENVT, Castanet-Tolosan, France
bioRxiv preprint
DOI: 10.1101/2021.03.09.434572