Shusuke Toden, Shigeyasu Kunitoshi, Jacob Cardenas, Jinghua Gu, Elizabeth Hutchins, Kendall Van Keuren-Jensen, Hiroyuki Uetake, Yuji Toiyama, and Ajay Goel
This study evaluates cancer stems cells (CSC) which resist chemotherapy agents and have high tumor initiation capacity. A particular gene variant within these cells, CD44, appears to present a more aggressive phenotype when over expressed. The researchers undertook miRNA profling of the CD44 in a cohort of patients to identify the dysregulated species. They suggest that these miRNAs could be used as prognostic biomarkers or possibly therapeutic targets for patients with colorectal cancer.
For this study, generated several CSC cell lines. ThePippinHT was used to isolate miRNA libraries (148bp).
Baylor Scott & White Research Institute and Charles A. Sammons Cancer Center. Dallas
Baylor Institute for Immunology Research, Dallas
Translational Genomics Research Institute, Phoenix, Arizona
Tokyo Medical and Dental University, Tokyo
Mie University Graduate School of Medicine, Mie, Japan
Journal of Clinical Investigation Insight