Assessing a single targeted next generation sequencing for human leukocyte antigen typing protocol for interoperability, as performed by users with variable experience

October 2017

Manish Gandhi, Deborah Ferriola, Curt Lind, Jamie Duke, et al.

Scientists from the Mayo Clinic, Children’s Hospital of Philadelphia, and other institutions teamed up to develop a streamlined, NGS-based HLA typing protocol. This method uses the Illumina MiSeq and size selection with the BluePippin. Samples were typed in six different labs, demonstrating high accuracy and reproducibility of results.

Human Immunology
Volume 78, Issue 10, Pages 642-648

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