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Recent Posts
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- In vitro size-selection of short circulating tumor DNA fragments from late-stage lung cancer patients enhance the detection of mutations and aneuploidies
- The haplotype-resolved T2T carnation (Dianthus caryophyllus) genome reveal the correlation between genome architecture and gene expression
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Author Archives: Alex
Moving to the Clinic: Interview with BC Cancer Agency’s Marco Marra
Mendelspod’s recent interview with Marco Marra of the BC Cancer Agency and the University of British Columbia is well worth a listen. In the podcast, Marra describes his team’s use of genome and transcriptome sequencing for patients whose cancer is … more »
Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization
December 2016 Authors: Peter M. Clark, Jamie L. Duke, Deborah Ferriola, Valia Bravo-Egana, Tunde Vago, Aniqa Hassan, Anna Papazoglou, and Dimitri Monos Info: Scientists from The Children’s Hospital of Philadelphia evaluated an NGS-based HLA typing kit from Omixon. They analyzed … more »
Posted in Citation
Tagged HLA, MiSeq, Pippin Prep
Comments Off on Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization
Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path
November 2016 Authors: Sandra C. Lorenz, Steven R. Monday, Maria Hoffmann, Markus Fischer and Julie A. Kase Info: Scientists from the US FDA and the University of Hamburg used PacBio sequencing to characterize plasmids associated with heightened virulence in Shiga … more »
Posted in Citation
Tagged BluePippin, PacBio
Comments Off on Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path
Very Short Mitochondrial DNA Fragments and Heteroplasmy in Human Plasma
November 2016 Authors: Ruoyu Zhang, Kiichi Nakahira, Xiaoxian Guo, Augustine M.K. Choi & Zhenglong Gu Info: Scientists at Cornell University studied cell-free mitochondrial DNA found in plasma samples collected from sepsis patients. Along the way, they optimized sample prep protocols … more »
Posted in Citation
Tagged BluePippin
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Preprints Galore!
We normally wait until papers come out in scientific journals before reporting on them here, but there are so many great preprints featuring Sage Science tools we couldn’t resist pointing them out. (On a side note, the rising numbers of … more »
Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
November 2016 Authors: Alan Van Goethem, Nurten Yigit, Celine Everaert, Myrthala Moreno-Smith, et al. Info: Scientists at Ghent University and Baylor College of Medicine evaluated protocols for depleting abundant RNAs from blood and serum samples to improve the ability to … more »
Posted in Citation
Tagged microRNA, Pippin Prep
Comments Off on Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
November 2016 Authors: Eileen P Hamilton, Aurélie Kapusta, Piroska E Huvos, Shelby L Bidwell, et al. Info: A large team of scientists in Asia, Europe, and the U.S. collaborated for this study of Tetrahymena thermophile, for which they produced a complete sequence assembly. They … more »
Posted in Citation
Tagged HiSeq, Pippin Prep
Comments Off on Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
Nanopore Sequencers Give Optimal Results with BluePippin Size Selection
The Oxford Nanopore team has been speaking recently about their use of our BluePippin automated size selection system for optimizing the read length obtained from nanopore sequencers. For anyone interested in the Oxford platforms who hasn’t seen this information, here’s … more »
Posted in Blog
Tagged BluePippin, ONT
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ASHG 2016: Structural Variants, Mega Databases, and the Best-Ever Human Genome
The Sage Science team enjoyed our trip across the border to Canada for the annual meeting of the American Society of Human Genetics this month. Vancouver was practically teeming with genome scientists! Many presentations this year featured results generated by … more »
Get a Glimpse of the New HLS Instrument at ASHG 2016
The Sage Science team is heading to Vancouver this week for the annual meeting of the American Society of Human Genetics. ASHG is the biggest genomics meeting we attend each year, and it never fails to deliver on its promise … more »