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Recent Posts
- New BluePippin 2.5% Agarose Cassettes Provide High Size Selection Accuracy Between 100-600bp
- New PippinHT Cassette: >10kB High-Pass with Internal Standards
- Improved circulating tumor DNA profiling by simultaneous extraction of DNA methylation and copy number information on from Methylated DNA Sequencing data (MeD-seq)
- Decoding Wild Rice Genomes: A New Era for Climate-Resilient Agriculture
- Chasing non-existent “microRNAs” in cancer
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Author Archives: Alex
New BluePippin 2.5% Agarose Cassettes Provide High Size Selection Accuracy Between 100-600bp
For Illumina and short-read sequencing applications, BluePippin users typically use either high-resolution 3% agarose gel cassettes and/or slightly lower resolution 2% agarose gel cassettes. The 3% cassettes are great for “threading the needle” when separating microRNA libraries from nearby unincorporated … more »
Posted in Applications, Blog
Tagged BluePippin
Comments Off on New BluePippin 2.5% Agarose Cassettes Provide High Size Selection Accuracy Between 100-600bp
New PippinHT Cassette: >10kB High-Pass with Internal Standards
Improved Workflow for HiFi Sequencing The PippinHT was designed to run two SBS-compatible gel cassettes, each with a 12-sample capacity, and with onboard sample tracking capability. In this way, DNA size selection could more closely match sample prep workflows undertaken … more »
Posted in Blog, Uncategorized
Comments Off on New PippinHT Cassette: >10kB High-Pass with Internal Standards
Improved circulating tumor DNA profiling by simultaneous extraction of DNA methylation and copy number information on from Methylated DNA Sequencing data (MeD-seq)
May 2025 Authors: Daan M Hazelaar, Ruben G Boers, Joachim B Boers, Vanja de Weerd, Jean Helmijr, Maurice PHM Jansen, Henk MW Verheul, Cornelis Verhoef, Joost Gribnau, John WM Martens, Stavros Makrodimtris, Saskia M Wilting Abstract: Cell-free DNA (cfDNA) analysis … more »
Decoding Wild Rice Genomes: A New Era for Climate-Resilient Agriculture
Asian rice, domesticated around 10,000 years ago, is a dietary staple for more than one third of the global population. Yet, intensive breeding for improved yield has reduced its genetic diversity – making modern varieties increasingly vulnerable to stressors like … more »
Posted in Blog, Uncategorized
Tagged BluePippin, CLR, HiFi, PacBio, PippinHT, Plant, Range+T
Comments Off on Decoding Wild Rice Genomes: A New Era for Climate-Resilient Agriculture
Chasing non-existent “microRNAs” in cancer
April 2025 Authors: Ayla Orang, Nicholas I. Warnock, Melodie Migault, B. Kate Dredge, Andrew G. Bert, Julie M. Bracken, Philip A. Gregory, Katherine A. Pillman, Gregory J. Goodall & Cameron P. Bracken Abstract: “MicroRNAs (miRNAs) are important regulators of gene … more »
Posted in Citation
Tagged cancer, miRNA, Pippin Prep
Comments Off on Chasing non-existent “microRNAs” in cancer
Telomere-to-telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa-pastoris (Brassicaceae)
March 2025 Authors: Xin Liu, Lu Liu, Hai-Fei Yan, Xue-Jun Ge, Hui-Run Huang Abstract: “Capsella is a model genus for studying polyploid speciation and mating system transitions. Here, we report a subgenome-resolved telomere-to-telomere reference genome of Capsella bursa-pastoris, the only … more »
Posted in Citation
Tagged HMW DNA, oxford nanopore, Plant, SageHLS
Comments Off on Telomere-to-telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa-pastoris (Brassicaceae)
Integrated analysis of miRNAs and mRNAs in thousands of single cells
January 2025 Authors: Jia Li, Jing Tian & Tao Cai Abstract: “The simultaneous sequencing of multiple types of biomolecules can facilitate understanding various forms of regulation occurring in cells. Cosequencing of miRNA and mRNA at single-cell resolution is challenging, and … more »
Posted in Citation
Tagged miRNA, Pippin Prep, RNA-seq
Comments Off on Integrated analysis of miRNAs and mRNAs in thousands of single cells
Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)
January 2025 Authors: Zhiqiang Zhang, Shujing He, Taoli Ding, Xiaoyan Liang, Cong Fang, Haitao Zeng, Linan Xu, Xiaolan Li, Lei Jia, Shihui Zhang, Wenlong Su, Peng Sun, Ji Yang, Jun Ren, Sijia Lu, Zi Ren Abstract: “Genetic diseases encompass a … more »
Posted in Citation
Tagged Adaptive Sequencing, BluePippin, ONT, oxford nanopore
Comments Off on Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)
CFTR haplotype phasing using long-read genome sequencing from ultra-low input DNA
January 2025 Authors: Neeru Gandotra, Antariksh Tyagi, Irina Tikhonova, Caroline Storer, Curt Scharfe Abstract: “Purpose: Newborn screening (NBS) identifies rare diseases that result from the recessive inheritance of pathogenic variants in both copies of a gene. Long-read genome sequencing (LRS) … more »
Integrated analysis of miRNAs and mRNAs in thousands of single cells
January 2025 Authors: Jia Li, Jing Tian, Tao Cai Abstract: “The simultaneous sequencing of multiple types of biomolecules can facilitate understanding various forms of regulation occurring in cells. Cosequencing of miRNA and mRNA at single-cell resolution is challenging, and to … more »
Posted in Citation
Tagged miRNA, mRNA, Pippin Prep, single cell sequencing
Comments Off on Integrated analysis of miRNAs and mRNAs in thousands of single cells