Author Archives: Alex

Decoding Wild Rice Genomes: A New Era for Climate-Resilient Agriculture

Posted on May 8, 2025 by Alex

Asian rice, domesticated around 10,000 years ago, is a dietary staple for more than one third of the global population. Yet, intensive breeding for improved yield has reduced its genetic diversity – making modern varieties increasingly vulnerable to stressors like … more »

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Chasing non-existent “microRNAs” in cancer

Posted on April 30, 2025 by Alex

April 2025 Authors: Ayla Orang, Nicholas I. Warnock, Melodie Migault, B. Kate Dredge, Andrew G. Bert, Julie M. Bracken, Philip A. Gregory, Katherine A. Pillman, Gregory J. Goodall & Cameron P. Bracken Abstract: “MicroRNAs (miRNAs) are important regulators of gene … more »

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Telomere-to-telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa-pastoris (Brassicaceae)

Posted on March 30, 2025 by Alex

March 2025 Authors: Xin Liu, Lu Liu, Hai-Fei Yan, Xue-Jun Ge, Hui-Run Huang Abstract: “Capsella is a model genus for studying polyploid speciation and mating system transitions. Here, we report a subgenome-resolved telomere-to-telomere reference genome of Capsella bursa-pastoris, the only … more »

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Integrated analysis of miRNAs and mRNAs in thousands of single cells

Posted on January 30, 2025 by Alex

January 2025 Authors: Jia Li, Jing Tian & Tao Cai Abstract: “The simultaneous sequencing of multiple types of biomolecules can facilitate understanding various forms of regulation occurring in cells. Cosequencing of miRNA and mRNA at single-cell resolution is challenging, and … more »

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Cost-effective and Flexible Preimplantation Genetic Testing (PGT) Using Adaptive Sampling-based Targeted Nanopore Sequencing (ASTN-seq)

Posted on January 21, 2025 by Alex

January 2025 Authors: Zhiqiang Zhang, Shujing He, Taoli Ding, Xiaoyan Liang, Cong Fang, Haitao Zeng, Linan Xu, Xiaolan Li, Lei Jia, Shihui Zhang, Wenlong Su, Peng Sun, Ji Yang, Jun Ren, Sijia Lu, Zi Ren Abstract: “Genetic diseases encompass a … more »

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CFTR haplotype phasing using long-read genome sequencing from ultra-low input DNA

Posted on January 21, 2025 by Alex

January 2025 Authors: Neeru Gandotra, Antariksh Tyagi, Irina Tikhonova, Caroline Storer, Curt Scharfe Abstract: “Purpose: Newborn screening (NBS) identifies rare diseases that result from the recessive inheritance of pathogenic variants in both copies of a gene. Long-read genome sequencing (LRS) … more »

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Integrated analysis of miRNAs and mRNAs in thousands of single cells

Posted on January 21, 2025 by Alex

January 2025 Authors: Jia Li, Jing Tian, Tao Cai Abstract: “The simultaneous sequencing of multiple types of biomolecules can facilitate understanding various forms of regulation occurring in cells. Cosequencing of miRNA and mRNA at single-cell resolution is challenging, and to … more »

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Leveraging the power of long reads for targeted sequencing

Posted on December 17, 2024 by Alex

December 2024 Authors: Shruti V. Iyer, Sara Goodwin, and William Richard McCombie Abstract: “Long-read sequencing technologies have improved the contiguity and, as a result, the quality of genome assemblies by generating reads long enough to span and resolve complex or … more »

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Resolving the 22q11.2 deletion using CTLR-Seq reveals chromosomal rearrangement mechanisms and individual variance in breakpoints

Posted on July 21, 2024 by Alex

July 2024 Authors: Bo Zhou, Carolin Purmann, Hanmin Guo, GiWon Shin, Yiling Huang, Reenal Pattni, Qingxi Meng, Stephanie U. Greer, Tanmoy Roychowdhuryg, Raegan N. Wood, Marcus Ho, Heinrich zu Dohna, Alexej Abyzov, Joachim F. Hallmayer, Wing H. Wong, Hanlee P. … more »

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CTLR-Seq Protocol

Posted on July 20, 2024 by Alex

July 2024 Authors: Bo Zhou, GiWon Shin,Yiling Huang, Raegan N. Wood, Hanlee P. Ji, Alexander E. Urban Abstract: “We developed a generally applicable method CRISPR/Cas9-targeted long read sequencing (CTLR-Seq) to resolve, haplotype-specifically, and at base-pair resolution, large, complex, and highly … more »

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