Monthly Archives: September 2017

It’s Baaack! Festival of Genomics Returns to Beantown

Posted on September 28, 2017 by Alex

It’s time for a genomics party! The Festival of Genomics returns to Boston next week, and we’re already looking forward to it. Sage is proud to be a sponsor and exhibitor at this great event, which brings together more than … more »

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Micro RNA’s (miRNA’s) may help explain expression of multiple genes in Alzheimer’s Frontal Cortex

Posted on September 20, 2017 by Alex

September 2017 Authors: James Bennett and Paula Keeney Info: Scientists from Neurodegeneration Therapeutics used RNA-seq to compare microRNAs found in Alzheimer’s brain samples and controls. They identified 55 up-regulated genes and 191 down-regulated genes in the Alzheimer’s samples. PippinHT was … more »

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Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method

Posted on September 18, 2017 by Alex

September 2017 Authors: Kengo Kato, Masanori Hashino, Tamaki Ito, Mari Matsui, et al. Info: Researchers developed and validated a streamlined protocol for PacBio sequencing sample prep. The method involves bead-beating for DNA isolation. For the project, the team used Pippin … more »

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Single molecule counting and assessment of random molecular tagging errors with transposable giga-scale error-correcting barcodes

Posted on September 18, 2017 by Alex

September 2017 Authors: Billy Lau and Hanlee Ji Info: Scientists aimed to address errors that occur with conventional molecular barcodes for RNA-seq workflows. They developed transposable error-correcting barcodes with tens of billions of unique labels and found that this approach … more »

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What’s the difference between the 4-10 Kb vs2 and 6-10 Kb vs3 high pass protocols?

Posted on September 15, 2017 by Alex

Customers may have noticed two very similar cassette definitions; 0.75%DF Marker S1 high-pass 4-10kb vs2 and 0.75%DF Marker S1 High-Pass 6-10kb vs3. Since these two protocols cover a similar range, they might seem a bit redundant. However, both of these … more »

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Scientists Compare PacBio, Oxford Nanopore Transcriptome Results

Posted on September 14, 2017 by Alex

We enjoy a good technology evaluation as much as the next scientist, particularly when it comes to sequencing. So we were quite interested in a recent F1000Research publication about long-read sequencing platforms from researchers at the University of Iowa, the … more »

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Complete Genome Sequences of Seven Strains Composing a Model Bacterial Community of Maize Roots

Posted on September 8, 2017 by Alex

September 2017 Authors: Ben Niu and Roberto Kolter Info: Scientists from Harvard Medical School released the full genome sequences of seven bacterial species they chose for a model community to understand microbial effects on the growth of maize root. They … more »

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Podcast: Nanopore Expert Mark Akeson on Challenges and Opportunities in Sequencing

Posted on September 5, 2017 by Alex

Mark Akeson knows a thing or two about perseverance. After spending years as a soil biologist in Guatemala where he endured a series of parasite infections, he went on to become a pioneer in nanopore sequencing technology, which he has … more »

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