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Monthly Archives: December 2016
Farewell to 2016
As 2016 draws to a close, we’re taking a look back to capture some of the highlights of the year before it disappears into the blur of previous years. One of the most exciting advances this year came from the … more »
Posted in Blog
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HLA Typing Workflow Uses Pippin Prep for Size Selection
We’ve written before about the shift toward NGS-based technologies for the HLA typing market. HLA typing is used for everything from understanding autoimmune and infectious diseases to matching organ transplants to recipients. But the HLA locus really make scientists and … more »
Posted in Blog
Tagged HLA, Pippin Prep
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Moving to the Clinic: Interview with BC Cancer Agency’s Marco Marra
Mendelspod’s recent interview with Marco Marra of the BC Cancer Agency and the University of British Columbia is well worth a listen. In the podcast, Marra describes his team’s use of genome and transcriptome sequencing for patients whose cancer is … more »
Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization
December 2016 Authors: Peter M. Clark, Jamie L. Duke, Deborah Ferriola, Valia Bravo-Egana, Tunde Vago, Aniqa Hassan, Anna Papazoglou, and Dimitri Monos Info: Scientists from The Children’s Hospital of Philadelphia evaluated an NGS-based HLA typing kit from Omixon. They analyzed … more »
Posted in Citation
Tagged HLA, MiSeq, Pippin Prep
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Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path
November 2016 Authors: Sandra C. Lorenz, Steven R. Monday, Maria Hoffmann, Markus Fischer and Julie A. Kase Info: Scientists from the US FDA and the University of Hamburg used PacBio sequencing to characterize plasmids associated with heightened virulence in Shiga … more »
Posted in Citation
Tagged BluePippin, PacBio
Comments Off on Plasmids from Shiga Toxin-Producing Escherichia coli Strains with Rare Enterohemolysin Gene (ehxA) Subtypes Reveal Pathogenicity Potential and Display a Novel Evolutionary Path
Very Short Mitochondrial DNA Fragments and Heteroplasmy in Human Plasma
November 2016 Authors: Ruoyu Zhang, Kiichi Nakahira, Xiaoxian Guo, Augustine M.K. Choi & Zhenglong Gu Info: Scientists at Cornell University studied cell-free mitochondrial DNA found in plasma samples collected from sepsis patients. Along the way, they optimized sample prep protocols … more »
Posted in Citation
Tagged BluePippin
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Preprints Galore!
We normally wait until papers come out in scientific journals before reporting on them here, but there are so many great preprints featuring Sage Science tools we couldn’t resist pointing them out. (On a side note, the rising numbers of … more »
Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples
November 2016 Authors: Alan Van Goethem, Nurten Yigit, Celine Everaert, Myrthala Moreno-Smith, et al. Info: Scientists at Ghent University and Baylor College of Medicine evaluated protocols for depleting abundant RNAs from blood and serum samples to improve the ability to … more »
Posted in Citation
Tagged microRNA, Pippin Prep
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Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome
November 2016 Authors: Eileen P Hamilton, Aurélie Kapusta, Piroska E Huvos, Shelby L Bidwell, et al. Info: A large team of scientists in Asia, Europe, and the U.S. collaborated for this study of Tetrahymena thermophile, for which they produced a complete sequence assembly. They … more »
Posted in Citation
Tagged HiSeq, Pippin Prep
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