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Tag Archives: PacBio
Genomics of Corynebacterium striatum, an emerging multidrug-resistant pathogen of immunocompromised patients
January 2018 Authors: K. Nudel, X. Zhao, S. Basu, X. Dong, et al. Info: Scientists from Harvard Medical School and other institutions used whole genome sequencing and MALDI/TOF to analyze seven C. striatum infections, all resistant to multiple drugs. They … more »
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Tagged BluePippin, PacBio
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Isoform Sequencing and State-of-Art Applications for Unravelling Complexity of Plant Transcriptomes
January 2018 Authors: Dong An, Hieu X. Cao, Changsheng Li, Klaus Humbeck and Wenqin Wang Info: In this paper, researchers offer best practices for using the Iso-Seq method with PacBio sequencing to analyze plant transcriptomes. They recommend BluePippin for size … more »
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Characterization of plasmids harboring blaCTX-M and blaCMY genes in E. coli from French broilers
January 2018 Authors: Fabrice Touzain, Laetitia Le Devendec, Claire de Boisséson, Sandrine Baron, et al. Info: In this study of avian E. coli collected from slaughterhouses or diseased broilers, scientists analyzed resistance to extended-spectrum cephalosporins. Sequencing was performed with Illumina … more »
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Tagged BluePippin, PacBio
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New Mosquito Assembly to Accelerate Science and Insect Control
There’s a lot to love about the new preprint announcing the first-ever truly high-quality reference genome assembly for the Aedes aegypti mosquito. The large consortium of scientists — the paper features a total of 72 co-authors — came together on … more »
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Tagged BluePippin, PacBio, SageELF
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Preprint Roundup: Long-Read Sequencing, ddRAD-seq, and a New Look at Viruses
We always keep our eyes peeled for interesting new research from scientists using Sage Science automated DNA size selection instruments, and several recent preprints caught our attention. Here’s a look: Nanopore Sequencing Reveals High-Resolution Structural Variation in the Cancer Genome … more »
Posted in Blog
Tagged BluePippin, ddRADseq, ONT, PacBio
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Long-read sequencing of the coffee bean transcriptome reveals the diversity of full-length transcripts
November 2017 Authors: Bing Cheng, Agnelo Furtado, Robert Henry Info: In this study, researchers generated the transcriptome of the tetraploid Arabica coffee bean, focusing on caffeine and sucrose genes for in-depth analysis. They used the PacBio Iso-Seq method with BluePippin … more »
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Scientists Compare PacBio, Oxford Nanopore Transcriptome Results
We enjoy a good technology evaluation as much as the next scientist, particularly when it comes to sequencing. So we were quite interested in a recent F1000Research publication about long-read sequencing platforms from researchers at the University of Iowa, the … more »
Complete Genome Sequences of Seven Strains Composing a Model Bacterial Community of Maize Roots
September 2017 Authors: Ben Niu and Roberto Kolter Info: Scientists from Harvard Medical School released the full genome sequences of seven bacterial species they chose for a model community to understand microbial effects on the growth of maize root. They … more »
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Dual redundant sequencing strategy: Full-length gene characterisation of 1056 novel and confirmatory HLA alleles
August 2017 Authors: Albrecht V, Zweiniger C, Surendranath V, Lang K, et al. Info: Scientists in Germany describe a high-throughput approach for characterizing novel HLA alleles. The method involves sequencing the full gene with PacBio and MiSeq. For PacBio sequencing, … more »
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Tagged BluePippin, HLA, MiSeq, PacBio
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Transcriptome Profiling Using Single-Molecule Direct RNA Sequencing Approach for In-depth Understanding of Genes in Secondary Metabolism Pathways of Camellia sinensis
July 2017 Authors: Qingshan Xu, Junyan Zhu, Shiqi Zhao, Yan Hou, et al. Info: Scientists from Anhui Agricultural University in China performed long-read transcriptome sequencing of the tea plant Camellia sinensis to better understand its production of flavonoids, theanine, and … more »
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Tagged BluePippin, PacBio, transcriptome
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