Tag Archives: DNA Sequencing

Synthetic Spike-in Standards Improve Run-Specific Systematic Error Analysis for DNA and RNA Sequencing

July 2012 Authors: Justin M. Zook, Daniel Samarov, Jennifer McDaniel, Shurjo K. Sen, Marc Salit Info: New approach to discovery of systematic sequencing errors by use of spike in RNA or DNA control DNAs. Pippin Prep used for RNA-seq library … more »

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Broad Institute Teams with Sage Science for Automated Sizing

Don’t miss this great blog post from the Broad Institute (“A Sage partnership”) describing collaborative work between their genome sequencing team and Sage Science to design a better size selection process for the Broad’s sequencing pipeline. Headed up by Sheila … more »

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At Emory, Size Selection Saves Time for NGS Prep

At the genome center at Emory University, scientists credit the Pippin Prep with shaving almost a full day off the sample prep process for Illumina’s mate-pair library prep. Chad Haase, laboratory manager at the Emory GRA Genome Center, says that … more »

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Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression

March 2012 Authors: Linzhao Cheng, Nancy F. Hansen, Ling Zhao, Yutao Du, Chunlin Zou, Frank X. Donovan, Bin-Kuan Chou, Guangyu Zhou, Shijie Li, Sarah N. Dowey, Zhaohui Ye, NISC Comparative Sequencing Program, Settara C. Chandrasekharappa, Huanming Yang, James C. Mullikin, … more »

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Mutation discovery in mice by whole exome sequencing

December 2011 Authors: Heather Fairfield, Griffith J Gilbert, Mary Barter, Rebecca R Corrigan, Michelle Curtain, Yueming Ding, Mark D’Ascenzo, Daniel J Gerhardt, Chao He, Wenhui Huang, Todd Richmond, Lucy Rowe, Frank J Probst, David E Bergstrom, Stephen A Murray, Carol … more »

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Molecular characterization of the translocation breakpoints in the Down syndrome mouse model Ts65Dn

September 2011 Authors: Laura G. Reinholdt, Yueming Ding, Griffith T. Gilbert, Anne Czechanski, Jeffrey P. Solzak, Randall J. Roper, Mark T. Johnson, Leah Rae Donahue, Cathleen Lutz, Muriel T. Davisson Info: Illumina PE libraries were constructed and size selected at … more »

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