Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome

March 2019

Jared P. Steranka, Zuojian Tang, Mark Grivainis, Cheng Ran Lisa Huang, Lindsay M. Payer, Fernanda O. R. Rego, Thiago Luiz Araujo Miller, Pedro A. F. Galante, Sitharam Ramaswami, Adriana Heguy, David Fenyö, Jef D. Boeke & Kathleen H. Burns

A new technique is described; Transposon Insertion Profiling (TIPseq). The method uses vectorette PCR and paired-end Illumina sequencing to map (LINE-1, L1) retrotransposon insertions in the human genome. A detailed protocol is provided.

Pippin Prep is used to remove fragments below 400 bp from libraries prior to sequencing.

Author Affiliations:
Johns Hopkins University School of Medicine, Baltimore, MD
NYU Langone Health, New York, NY,
Hospital Sírio-Libanês, São Paulo, Brazil
Universidade de São Paul, São Paulo, Brazil

Mobile DNA
DOI: 10.1186/s13100-019-0148-5

This entry was posted in Citation and tagged , , , . Bookmark the permalink.

Comments are closed.