Charles Y. Lin, Jakob Loven,Peter B. Rahl, Ronald M. Paranal, Christopher B. Burge, James E. Bradner, Tong Ihn Lee, and Richard A. Young
This high impact study completely revises knowledge on how c-myc gene is involved with gene regulation and cancer. Materials and Methods: Pippin Prep was used for preparation of ChIP-seq. Used multiplexed TruSeq v2 kits. IPDNA end-repaired and A tailed, ligated to adapters, enriched by 18 cycles PCR, then size selected on Pippin to obtain 200-400 bp fragments. Sequenced in single read mode 40b reads, Illumina HiSeq 2000.
Cell 151, 56–67, September 28, 2012