Re-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere

March 2017

Authors:
Henk J. Schouten, Henri vande Geest, Sofia Papadimitriou, Marian Bemer, Jan G. Schaart, Marinus J. M. Smulders, Gabino Sanchez Perez, Elio Schijlen

Info:
Scientists from Wageningen University and Research sequenced and analyzed several Arabidopsis thaliana plants to understand the mutagenic effects of Agrobacterium tumefaciens. As part of this project, they identified a 50 bp partial T-DNA insert and named it a T-DNA splinter. BluePippin was used to remove short fragments from the library prior to sequencing with PacBio.

Citation:
Plant Cell Reports, volume 36, Issue 3, pp 493–504
DOI: 10.1007/s00299-017-2098-z

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