Molecular indexing enables quantitative targeted RNA sequencing and reveals poor efficiencies in standard library preparations

January 2014

Glenn K. Fu, Weihong Xu, Julie Wilhelmy, Michael N. Mindrinos, Ronald W. Davis, Wenzhong Xiao, and Stephen P. A. Fodor

Scientists from Cellular Research and Stanford University describe a method for improving the use of RNA-seq for absolute quantification of gene expression by barcoding cDNA molecules. This molecular indexing method allowed the team to perform deep sampling and to quantify rare transcripts. Pippin Prep was used for sizing of the cDNA sample as well as for size selection in the digital PCR validation protocol.

PNAS 2014 111 (5) 1891–1896


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