Modified paired end rapid library preparation protocol for 454 GS Junior 8 kb library preparation using Covaris g-tubes and BluePippin electrophoresis

August 2014

Authors:
Janet Hill, Bonnie Chaban, Jennifer Town, Matthew Links & Tim Dumonceaux

Info:
The scientists report a guide for 454 sample prep for paired-end reads. In their method, they eliminate the need for several expensive pieces of lab equipment and use the BluePippin to perform DNA size selection. These adjustments make the process faster and less costly.

Citation:
Protocol Exchange
doi:10.1038/protex.2014.028

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