KRAS-dependent sorting of miRNA to exosomes

July 2015

Authors:
Diana J Cha, Jeffrey L Franklin, Yongchao Dou, Qi Liu, James N Higginbotham, Michelle Demory Beckler, Alissa M Weaver, Kasey Vickers, Nirpesh Prasad, Shawn Levy, Bing Zhang, Robert J Coffey, and James G Patton

Info:
Scientists from Vanderbilt University and the HudsonAlpha Institute for Biotechnology studied exosomes released by colorectal cancer cells with mutated KRAS, finding that exosomal miRNAs can function in target cells and that exosomal profiles are different from cellular profiles. Pippin Prep was used to select small RNAs, and sequencing was performed on an Illumina HiSeq.

Citation:
eLife 2015;4:e07197
DOI: http://dx.doi.org/10.7554/eLife.07197

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