Distinct Profiles of Cell-Free MicroRNAs in Plasma of Veterans with Post-Traumatic Stress Disorder

July 2019

Min Young Lee, David Baxter, Kelsey Scherler , Taek-Kyun Kim, Xiaogang Wu , Duna Abu-Amara, Janine Flory, Rachel Yehuda, Charles Marma, Marti Jett, Inyoul Lee, Kai Wang, and Leroy Hood
Researchers report on studies that profile circulating miRNAs in combat veterans suffering from Post Traumatic Stress Disorder (PTSD). Previous studies had shown that circulating miRNA spectra in extra cellular vesicles (EV) in patients suffering from psychiatric disorders. The authors confirm that miRNA profiles differ between PTSD and non-PTSD patients in both EV and EV-depleted fractions of plasma. Further study is recommended to understand the utility of these findings.
PippinHT was used to isolate modified Illumina small RNA sequencing libraries (134-162bp size selection for 20-22 bp inserts). The method (Giraldez et al. 2019) uses custom degenerate adapters (4N adapters) and increased polyethylene glycol (PEG) during the ligation process. This reduces bias and optimizes several other parameters to make it appropriate for use specifically with plasma RNA.

Author Affiliations:
Institute for Systems Biology, Seattle
Steven and Alexandra Cohen Veterans Center for Posttraumatic Stress and Traumatic Brain Injury, New York University, New York
Icahn School of Medicine at Mount Sinai, New York
James J. Peters VA Medical Center, New Yourk
Integrative Systems Biology, US Army Center for Environmental Health Research, Frederick, MD
Journal of Clinical Medicine
DOI: 10.3390/jcm8070963

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