Detection of individual ploidy levels with genotyping‐by‐sequencing (GBS) analysis

February 2017

Zachariah Gompert, Karen E. Mock

Utah State University researchers developed a statistical approach to detect low-level ploidy variants using genotyping data from a sequencing workflow. The method does not require prior ploidy information to function. An evaluation was performed on Populus tremuloides, in which it successfully distinguished diploids, triploids and tetraploids. For this work, BluePippin was used to select 300 bp – 400 bp fragments prior to sequencing.

Molecular Ecology Resources
DOI: 10.1111/1755-0998.12657

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