Detecting DNA cytosine methylation using nanopore sequencing

February 2017

Authors:
Jared Simpson, Rachael Workman, PC Zuzarte, Matei David, LJ Dursi & Winston Timp

Info:
In this paper, scientists report developing algorithms to distinguish methylated from unmethylated DNA using the Oxford Nanopore MinION sequencer. They evaluated its performance by sequencing a human methylome. BluePippin was used to size-select libraries for 3,500 bp to 6,000 bp.

Citation:
Nature Methods 14, 407–410 (2017)
doi:10.1038/nmeth.4184

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