De Novo Assembly of a New Solanum pennellii Accession Using Nanopore Sequencing

October 2017

Maximilian Schmidt, Alxander Vogel, Alisandra Denton, Benjamin Istace, et al.

Scientists used Oxford Nanopore technology to sequence Solanum pennellii, a wild species of tomato. They generated a median read length of nearly 12 Kb and produced an assembly with a contig N50 of 2.5 Mb. The high error rate was reduced by polishing with Illumina reads. The team enriched for long reads by using BluePippin to remove smaller fragments prior to sequencing.

The Plant Cell
doi: 10.1105/tpc.17.00521

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