PippinHT DNA Size Selection System
Up to 24 samples per run. Selects 100 bp – 1.5 kb. High Pass up to 40 kb.
BluePippin DNA Size Selection System
Selects 100 bp – 50 kb. High Pass up to 40 kb.
Pippin Prep DNA Size Selection System
Selects 100 bp – 1.5 kb.
HLS2 HMW Library System
Cas9 target selection of large genomic regions. HMW DNA extraction.
SageELF Whole Sample Fractionation System
DNA (up to 40 kb) or Proteins (up to 500 kDa).
Pippin Pulse Electrophoresis Power Supply
Resolves DNA up to 400kb on a midi gel box.
TELL-Seq™ Bar Code Linked-read Library Kit
Transposase insertion of bar-codes into NGS libraries
July 2015
Authors:
Darren Heavens, Gonzalo Garcia Accinelli, Bernardo Clavijo, and Matthew Derek Clark
Info:
Scientists from The Genome Analysis Centre report a protocol using the SageELF to improve library preparation for long mate-pair sequencing. Using a Nextera kit, they show that generating 12 fractions with SageELF saves money and time while requiring less DNA input compared to other gel-based sizing options.
Citation:
BioTechniques 59:42-45 (July 2015)
DOI: 10.2144/000114310