Category Archives: Blog

What’s the difference between the 4-10 Kb vs2 and 6-10 Kb vs3 high pass protocols?

Customers may have noticed two very similar cassette definitions; 0.75%DF Marker S1 high-pass 4-10kb vs2 and 0.75%DF Marker S1 High-Pass 6-10kb vs3. Since these two protocols cover a similar range, they might seem a bit redundant. However, both of these … more »

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Scientists Compare PacBio, Oxford Nanopore Transcriptome Results

We enjoy a good technology evaluation as much as the next scientist, particularly when it comes to sequencing. So we were quite interested in a recent F1000Research publication about long-read sequencing platforms from researchers at the University of Iowa, the … more »

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Podcast: Nanopore Expert Mark Akeson on Challenges and Opportunities in Sequencing

Mark Akeson knows a thing or two about perseverance. After spending years as a soil biologist in Guatemala where he endured a series of parasite infections, he went on to become a pioneer in nanopore sequencing technology, which he has … more »

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Nabsys Returns: CEO Barrett Bready on the Importance of Structural Variation

A new podcast from Mendelspod features an interesting interview with Barrett Bready, CEO of electronic mapping firm Nabsys, who emphasizes the growing need to incorporate structural variation data into genome studies. In the discussion, Bready describes his company’s platform, which … more »

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ddRAD-seq Study Explores Behavioral Roles in Speciation

A new preprint from the Hoekstra lab at Harvard makes great use of the double digest RAD-seq protocol to better understand reproductive barriers and speciation in closely related species of mice. Since it was the Hoekstra lab that gave us … more »

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For 10x Genomics Workflow, Broad Institute Uses PippinHT Size Selection

At the Broad Institute, scientist Michelle Cipicchio is part of the technology development team responsible for optimizing new methods or sample types before they’re implemented on the organization’s industrial-scale exome and whole-genome sequencing pipeline. Recently, she’s been working with the … more »

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PacBio Users: Size Selection Is Essential for Generating Excellent Results

The Sage Science team was delighted to attend and co-sponsor PacBio’s annual East Coast user group meeting in Baltimore last week, particularly since there was a half-day session devoted to our favorite subject: sample prep. There were plenty of customer … more »

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Preprint Highlights Utility of Size Selection for Nanopore Studies

A recently shared preprint demonstrates the effectiveness of size-selection for nanopore sequencing, relying on the PippinHT automated DNA sizing platform for high-throughput pipelines. “Mapping And Phasing Of Structural Variation In Patient Genomes Using Nanopore Sequencing” comes from lead author Mircea … more »

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Creighton Lab Boosts Yield, Sequencing Efficiency with BluePippin

At Creighton University in Omaha, Neb., Dr. Anna Selmecki’s lab explores various fungal species to understand genome instability, pathogenesis, and the acquisition of drug resistance. For these investigations, her team relies heavily on whole genome sequencing, using both the Illumina … more »

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How to CATCH Your Gene of Interest

If you haven’t heard about CATCH by now, it’s time to catch up. Short for Cas9-assisted targeting of chromosome segments, CATCH comes from the lab of Yuval Ebenstein at Tel Aviv University and was first reported in this Nature Communications … more »

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