Category Archives: Blog

Podcast: Nanopore Expert Mark Akeson on Challenges and Opportunities in Sequencing

Posted on September 5, 2017 by Alex

Mark Akeson knows a thing or two about perseverance. After spending years as a soil biologist in Guatemala where he endured a series of parasite infections, he went on to become a pioneer in nanopore sequencing technology, which he has … more »

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Nabsys Returns: CEO Barrett Bready on the Importance of Structural Variation

Posted on July 27, 2017 by Alex

A new podcast from Mendelspod features an interesting interview with Barrett Bready, CEO of electronic mapping firm Nabsys, who emphasizes the growing need to incorporate structural variation data into genome studies. In the discussion, Bready describes his company’s platform, which … more »

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ddRAD-seq Study Explores Behavioral Roles in Speciation

Posted on July 25, 2017 by Alex

A new preprint from the Hoekstra lab at Harvard makes great use of the double digest RAD-seq protocol to better understand reproductive barriers and speciation in closely related species of mice. Since it was the Hoekstra lab that gave us … more »

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For 10x Genomics Workflow, Broad Institute Uses PippinHT Size Selection

Posted on July 18, 2017 by Alex

At the Broad Institute, scientist Michelle Cipicchio is part of the technology development team responsible for optimizing new methods or sample types before they’re implemented on the organization’s industrial-scale exome and whole-genome sequencing pipeline. Recently, she’s been working with the … more »

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PacBio Users: Size Selection Is Essential for Generating Excellent Results

Posted on July 6, 2017 by Alex

The Sage Science team was delighted to attend and co-sponsor PacBio’s annual East Coast user group meeting in Baltimore last week, particularly since there was a half-day session devoted to our favorite subject: sample prep. There were plenty of customer … more »

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Preprint Highlights Utility of Size Selection for Nanopore Studies

Posted on June 29, 2017 by Alex

A recently shared preprint demonstrates the effectiveness of size-selection for nanopore sequencing, relying on the PippinHT automated DNA sizing platform for high-throughput pipelines. “Mapping And Phasing Of Structural Variation In Patient Genomes Using Nanopore Sequencing” comes from lead author Mircea … more »

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Creighton Lab Boosts Yield, Sequencing Efficiency with BluePippin

Posted on June 13, 2017 by Alex

At Creighton University in Omaha, Neb., Dr. Anna Selmecki’s lab explores various fungal species to understand genome instability, pathogenesis, and the acquisition of drug resistance. For these investigations, her team relies heavily on whole genome sequencing, using both the Illumina … more »

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How to CATCH Your Gene of Interest

Posted on May 18, 2017 by Alex

If you haven’t heard about CATCH by now, it’s time to catch up. Short for Cas9-assisted targeting of chromosome segments, CATCH comes from the lab of Yuval Ebenstein at Tel Aviv University and was first reported in this Nature Communications … more »

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On DNA Day, Let’s Stop the Slicing and Dicing

Posted on April 25, 2017 by Alex

Today is DNA Day, and we’re taking the opportunity to support the humane treatment of DNA. After all these years of harshly shearing these molecules and fragmenting them down to just a few hundred bases, can’t we agree that there … more »

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At AACR, High-Profile Speakers and NGS Error Correction in the Spotlight

Posted on April 10, 2017 by Alex

Last week’s annual meeting of the American Association for Cancer Research offered some great perspectives on innovation in oncology, both in the clinic and in academic labs. We were particularly impressed by former Vice President Joe Biden’s update on the … more »

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