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Category Archives: Blog
ASMS, Here We Come!
We are packing our bags for Minneapolis and the 61st annual conference of the American Society for Mass Spectrometry. As scientists who come from the DNA realm, the protein world is very exciting to us and we’re looking forward to … more »
New Prostate Cancer Research Indicates Rapid Accumulation of DNA Mutations
Researchers studying the behavior of prostate cancer have shed light on how the cancer develops mutations so rapidly. In contrast to other cancers that seem to progress by adding mutations one at a time, the prostate cancer samples that were … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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Pippin and PacBio: Better Together
Update: Just announced– Sage Science and Pacific Biosciences have partnered to offer users longer reads than ever. Click here to read the press release. The new SMRTbellTM 20kb sample prep protocol using the BluePippin is posted on the PacBio® SampleNet … more »
Posted in Blog
Tagged agbt, BluePippin, DNA Sequencing, PacBio
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Pippin Pulse
Field Inversion Gel Electrophoresis We’ve set up this post to provide Pippin Pulse users with a resource to keep updated on new protocols and to post references that might be useful. We also invite users to feel … more »
Posted in Blog, Tips and Applications
Tagged BluePippin, Pulse, Tips and Applications
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Improved Recovery for 3-10kb DNA Size Selection
BluePippin users, we have a new cassette definition that will appear in the Cassette Definition Set 10 (CD10) that we feel merits some further detail. This definition is named “0.75% DF 3 – 10kb Marker S1 – Improved Recovery” What … more »
At TGAC, Pippin-Aided Sequencing Gives Quality Boost to Genome Assemblies
Before a new technology is deployed at The Genome Analysis Centre in Norwich, UK, it must run the gauntlet of Matthew Clark’s lab. Clark is the sequencing technology development leader at TGAC, where part of his role involves testing out … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Illumina, Mate-pair Sequencing, PacBio, Pippin System
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NHGRI Scientists Find Transgenic DNA Has More Rearrangements than Expected
Amanda DuBose didn’t set out to come up with a new technical solution to characterizing transgenic DNA in animal models. A postdoctoral fellow in Francis Collins’ lab at the National Human Genome Research Institute, DuBose was using mice to study … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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At Iowa Core Lab, Pippin Sizing Is Essential for MicroRNA Studies
Kevin Knudtson’s DNA Facility at the University of Iowa acquired a Pippin Prep almost a year ago, and in the time since, the automated size selection tool has become an integral part of the core lab’s microRNA pipeline. “Pippin is … more »
Posted in Blog
Tagged ChIP-Seq, DNA Sequencing, miRNA isolation, Pippin Prep
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Pippin Shines in Size Selection Comparison from Sanger
A paper that came out December in the journal Electrophoresis compares Pippin Prep size selection with Caliper’s Labchip XT and finds that Pippin gives “very tight size distributions” not seen with other sizing methods. “Evaluation and optimisation of preparative semi-automated … more »
Posted in Blog
Tagged DNA Sequencing, Illumina, Pippin Prep
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At MIT, Pippin Enables Splice Variant Detection and MicroRNA Analysis
At the BioMicro Center at MIT, Director Stuart Levine, PhD, recently introduced the Pippin Prep from Sage Science to enable key applications — including splice variant analysis with RNA-seq and microRNA analysis — that were not possible on other platforms. … more »
Posted in Blog
Tagged miRNA isolation, Pippin Prep, RNA-seq
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