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Recent Posts
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- In vitro size-selection of short circulating tumor DNA fragments from late-stage lung cancer patients enhance the detection of mutations and aneuploidies
- The haplotype-resolved T2T carnation (Dianthus caryophyllus) genome reveal the correlation between genome architecture and gene expression
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Author Archives: Alex
Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
September 2017 Authors: Kengo Kato, Masanori Hashino, Tamaki Ito, Mari Matsui, et al. Info: Researchers developed and validated a streamlined protocol for PacBio sequencing sample prep. The method involves bead-beating for DNA isolation. For the project, the team used Pippin … more »
Posted in Citation
Tagged Pippin Pulse
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Single molecule counting and assessment of random molecular tagging errors with transposable giga-scale error-correcting barcodes
September 2017 Authors: Billy Lau and Hanlee Ji Info: Scientists aimed to address errors that occur with conventional molecular barcodes for RNA-seq workflows. They developed transposable error-correcting barcodes with tens of billions of unique labels and found that this approach … more »
Posted in Citation
Tagged Pippin Prep
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What’s the difference between the 4-10 Kb vs2 and 6-10 Kb vs3 high pass protocols?
Customers may have noticed two very similar cassette definitions; 0.75%DF Marker S1 high-pass 4-10kb vs2 and 0.75%DF Marker S1 High-Pass 6-10kb vs3. Since these two protocols cover a similar range, they might seem a bit redundant. However, both of these … more »
Posted in Blog, Tips
Tagged BluePippin, High Pass, Tips and Applications
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Scientists Compare PacBio, Oxford Nanopore Transcriptome Results
We enjoy a good technology evaluation as much as the next scientist, particularly when it comes to sequencing. So we were quite interested in a recent F1000Research publication about long-read sequencing platforms from researchers at the University of Iowa, the … more »
Complete Genome Sequences of Seven Strains Composing a Model Bacterial Community of Maize Roots
September 2017 Authors: Ben Niu and Roberto Kolter Info: Scientists from Harvard Medical School released the full genome sequences of seven bacterial species they chose for a model community to understand microbial effects on the growth of maize root. They … more »
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Tagged BluePippin, PacBio
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Podcast: Nanopore Expert Mark Akeson on Challenges and Opportunities in Sequencing
Mark Akeson knows a thing or two about perseverance. After spending years as a soil biologist in Guatemala where he endured a series of parasite infections, he went on to become a pioneer in nanopore sequencing technology, which he has … more »
A SINE insertion in ATP1B2 in Belgian Shepherd dogs affected by spongy degeneration with cerebellar ataxia (SDCA2)
August 2017 Authors: Nico Mauri, Miriam Kleiter, Elisabeth Dietschi, Michael Leschnik, et al. Info: Scientists in Europe and the US studied the genetic basis for a neurodegenerative disorder in dogs. Through whole genome sequencing data, they found a SINE insertion … more »
Nabsys Returns: CEO Barrett Bready on the Importance of Structural Variation
A new podcast from Mendelspod features an interesting interview with Barrett Bready, CEO of electronic mapping firm Nabsys, who emphasizes the growing need to incorporate structural variation data into genome studies. In the discussion, Bready describes his company’s platform, which … more »
Dual redundant sequencing strategy: Full-length gene characterisation of 1056 novel and confirmatory HLA alleles
August 2017 Authors: Albrecht V, Zweiniger C, Surendranath V, Lang K, et al. Info: Scientists in Germany describe a high-throughput approach for characterizing novel HLA alleles. The method involves sequencing the full gene with PacBio and MiSeq. For PacBio sequencing, … more »
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Tagged BluePippin, HLA, MiSeq, PacBio
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Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity
July 2017 Authors: Kelley Yan, Olivier Gevaert, Grace Zheng, Benedict Anchang, et al. Info: In this project from scientists at Stanford University School of Medicine and other institutions, comparative RNA-seq was used to analyze putative mouse intestinal stem cells. The … more »
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Tagged Pippin Prep, RNA-seq
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