December 2016
Authors:
Peter M. Clark, Jamie L. Duke, Deborah Ferriola, Valia Bravo-Egana, Tunde Vago, Aniqa Hassan, Anna Papazoglou, and Dimitri Monos
Info:
Scientists from The Children’s Hospital of Philadelphia evaluated an NGS-based HLA typing kit from Omixon. They analyzed 50 samples; 7.7% were found to have novel polymorphisms, suggesting that new sequencing approaches could usher in a new era of discovery for HLA alleles. The team used Pippin Prep to size-select libraries prior to sequencing on a MiSeq.
Citation:
Clinical Chemistry
DOI: 10.1373/clinchem.2016.260661