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Monthly Archives: August 2016
Comparative genomics and transcriptomics of Pichia pastoris
August 2016 Authors: Kerry R. Love, Kartik A. Shah, Charles A. Whittaker, Jie Wu, M. Catherine Bartlett, Duanduan Ma, Rachel L. Leeson, Margaret Priest, Jonathan Borowsky, Sarah K. Young and J. Christopher Love Info: To provide a foundation for engineering … more »
Posted in Citation
Tagged DNA Sequencing, HiSeq, Illumina, Pippin Prep
Comments Off on Comparative genomics and transcriptomics of Pichia pastoris
RADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data
August 2016 Authors: Hoffberg SL, Kieran TJ, Catchen JM, Devault A, Faircloth BC, Mauricio R, Glenn TC Info: In this methods paper, scientists combine double-digest RAD-seq with capture technology to reduce costs and increase efficiency. The protocol, which was validated … more »
Posted in Citation
Tagged ddRADseq, genotyping, Illumina, Methods, NextSeq, Pippin Prep, Target Enrichment
Comments Off on RADcap: sequence capture of dual-digest RADseq libraries with identifiable duplicates and reduced missing data
At the University of Delaware, Early Adopter Genomics Core Spots New Science Trends
The genomics core facility at the University of Delaware has set itself apart from other service providers by being among the first to adopt new sequencing technologies. The strategy has been a success: today, the facility serves customers around the … more »
Posted in Blog
Tagged BluePippin, customer, SageELF
Comments Off on At the University of Delaware, Early Adopter Genomics Core Spots New Science Trends
Complete Genome Sequence of a Klebsiella pneumoniae Strain Carrying blaNDM-1 on a Multidrug Resistance Plasmid
July/August 2016 Authors: Sean Conlan, Anna F. Lau, NISC Comparative Sequencing Program, Tara N. Palmore, Karen M. Frank, and Julia A. Segre Info: NIH scientists sequenced the genome of the first blaNDM-1-positive strain of Klebsiella pneumoniae detected in the institute’s hospital. They found three plasmids, … more »
Posted in Citation
Tagged BluePippin, PacBio
Comments Off on Complete Genome Sequence of a Klebsiella pneumoniae Strain Carrying blaNDM-1 on a Multidrug Resistance Plasmid
Circulating Extracellular RNA Markers of Liver Regeneration
July 2016 Authors: Irene K. Yan, Xue Wang, Yan W. Asmann, Hiroaki Haga, Tushar Patel Info: Mayo Clinic researchers performed partial hepatectomies in mice and then monitored RNA activity in serum samples to find a signature associated with liver regeneration. … more »
Posted in Citation
Tagged cfRNA, Illumina, MiSeq, Pippin Prep, RNA sequencing
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Analysis of tandem gene copies in maize chromosomal regions reconstructed from long sequence reads
July 2016 Authors: Jiaqiang Dong, Yaping Feng, Dibyendu Kumar, Wei Zhang, Tingting Zhu, Ming-Cheng Luo, and Joachim Messing Info: Scientists from Rutgers University and the University of California, Davis, analyzed copy number variation in the highly repetitive maize genome using … more »
Posted in Citation
Tagged BluePippin, High Pass, PacBio
Comments Off on Analysis of tandem gene copies in maize chromosomal regions reconstructed from long sequence reads
Genome-wide identification of enhancer elements
June 2016 Authors: Sarah Tulin, Julius C. Barsi, Carlo Bocconcelli, and Joel Smith Info: The authors (from Woods Hole and Cal Tech) present a genome-wide regulatory element database for the sea urchin embryo. To do this, they developed a new … more »
PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer
June 2016 Authors: Jennifer M. Spangle, Koen M. Dreijerink, Anna C. Groner, Hailing Cheng, Carolynn E. Ohlson, Jaime Reyes, Charles Y. Lin, James Bradner, Jean J. Zhao, Thomas M. Roberts, and Myles Brown Info: In this breast cancer research study, … more »
Posted in Citation
Tagged ChIP-Seq, Illumina, Pippin Prep
Comments Off on PI3K/AKT Signaling Regulates H3K4 Methylation in Breast Cancer