Single-Step Selection of Bivalent Aptamers Validated by Comparison with SELEX Using High-Throughput Sequencing

June 2014

Authors:
Robert Wilson, Christian Bourne, Roy R. Chaudhuri, Richard Gregory, John Kenny, Andrew Cossins

Info:
This study, conducted by researchers at the University of Liverpool, aimed at accelerating the identification of nucleic acid aptamers by finding ways to reduce the number of rounds of selection and amplification required. They determined that next-gen sequencing and motif-finding informatics both show promise for simplifying aptamer selection. Pippin Prep was used for DNA size selection at multiple points in this technical comparison.

Citation:
PLOS ONE 9(6): e100572. doi:10.1371/journal.pone.0100572

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