November 2012
Authors:
Terumi Kohwi-Shigematsu, Yoshinori Kohwi, Keiko Takahashi, Hunter W. Richards, Stephen D. Ayers, Hye-Jung Han, Shutao Cai
Info:
Among other things, this paper outlines methods for mapping all DNA binding sites for a global chromatin organizing protein, SATB1. Recombinant HIS-tagged SATB1 protein is incubated with sheared genomic DNA, SATB1 with bound DNA is captured on magnetic HIS particles, and then DNA libraries are prepared from the particle-bound DNA. Illumina library construction is used, with size selection on the Pippin Prep (150-500bp).
Citation:
Methods: November 2012