Clarisse Marotz, Jon Sanders, Cristal Zuniga, Livia Zaramela, Rob Knight, and Karsten Zengler
Scientists from the University of California, San Diego, developed and evaluated a new protocol for enriching microbial DNA collected from fresh and frozen human saliva samples. Compared to commercially available kits, their method is “cost-effective, rapid, and robust,” the team concludes. They used PippinHT to remove primer dimers and target 300 bp to 800 bp DNA fragments prior to sequencing.
Microbiome. 2018; 6: 42.