Improving saliva shotgun metagenomics by chemical host DNA depletion

February 2018

Clarisse Marotz, Jon Sanders, Cristal Zuniga, Livia Zaramela, Rob Knight, and Karsten Zengler

Scientists from the University of California, San Diego, developed and evaluated a new protocol for enriching microbial DNA collected from fresh and frozen human saliva samples. Compared to commercially available kits, their method is “cost-effective, rapid, and robust,” the team concludes. They used PippinHT to remove primer dimers and target 300 bp to 800 bp DNA fragments prior to sequencing.

Microbiome. 2018; 6: 42.

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