June 2018
Authors:
Joseph Cartwright, Karin Anderson, Joseph Longworth, Philip Lobb, and David James
Info:
To improve the quantitative assessment of recombinant DNA in cell lines, University of Sheffield scientists and collaborators developed a PacBio-based method for high-throughput rDNA sequence analysis. The protocol can sequence 40,000 individual recombinant DNA molecules at once to find point mutations. BluePippin was used for purification prior to sequencing.
Citation:
Biotechnology and Bioengineering
doi: 10.1002/bit.26561