Finding Nemo: Hybrid assembly with Oxford Nanopore and Illumina reads greatly improves the Clownfish (Amphiprion ocellaris) genome assembly

January 2018

Mun Hua Tan, Christopher Austin, Michael Hammer, Yin Peng Lee, Laurence Croft, Han Ming Gan

This paper presents the first hybrid genome assembly of Amphiprion ocellaris, the clown anemonefish. The team combined data from Illumina and Oxford Nanopore sequencing, reducing the number of scaffolds compared to a short-read-only assembly by 94%. BluePippin was used to select large DNA fragments for nanopore sequencing.

doi: 10.1093/gigascience/gix137

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