Error rates, PCR recombination, and sampling depth in HIV-1 whole genome deep sequencing

July 2017

Authors:
Fabio Zanini, Johanna Brodin, Jan Albert, Richard Neher

Info:

Researchers in Germany and Sweden expounded on a previous deep sequencing study of HIV-1 populations to evaluate challenges such as error profiles and proper controls. For the whole genome sequencing, they used BluePippin instead of bead-based sizing to remove inserts shorter than 350 bp to maximize read length on the Illumina MiSeq system.

Citation:
Virus Research, Volume 239, Pages 106-114
doi: 10.1016/j.virusres.2016.12.009

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