Complete telomere-to-telomere de novo assembly of the Plasmodium falciparum genome through long-read (>11 kb), single molecule, real-time sequencing

June 2016

Shruthi Sridhar Vembar, Matthew Seetin, Christine Lambert, Maria Nattestad, Michael C. Schatz, Primo Baybayan, Artur Scherf, and Melissa Laird Smith

Scientists in France and the US used PacBio sequencing to characterize the AT-rich genome of this deadly malaria parasite. Half of all sequence reads were longer than 15.5 Kb, allowing the scientists to produce an assembly with each chromosome fully resolved. They used BluePippin to eliminate fragments smaller than 15 Kb and Pippin Pulse to assess size distribution of sheared DNA.

DNA Res (2016)
doi: 10.1093/dnares/dsw022

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