February 2016
Authors:
Andrea D. Tyler, Sara Christianson, Natalie C. Knox, Philip Mabon, Joyce Wolfe, Gary Van Domselaar, Morag R. Graham, and Meenu K. Sharma
Info:
In this PLoS One publication, scientists in Manitoba compare the quality of libraries constructed with Illumina’s Nextera and TruSeq protocols for Mycobacterium tuberculosis, which has a particularly challenging genome. They determined that the TruSeq method yielded more uniform data quality and coverage. BluePippin was used for size selection prior to sequencing on a MiSeq.
Citation:
DOI: 10.1371/journal.pone.0148676