December 2012
Authors:
Josephine Chandler, Thao T. Truong, Patricia M. Silva, Mohammad R. Seyedsayamdost, Galvin Carr, Matthew Radey, Michael A. Jacobs, Elizabeth H. Sims, Jon Clardy, E. Peter Greenberg
Info:
Authors were searching for cause of resistance to antibiotic Bactobolin. Performed Illumina paired-end sequencing on resistant strains. Library construction used size-selection of library on Pippn Prep. Discovery of mutation was straightforward for two of four strains. Other two strains harbored a three base insertion which was not recognized in the initial Illumina sequencing.
Citation:
mBio 3(6):e00499-12: December 2012