Assessing the utility of whole genome amplified DNA for next-generation molecular ecology

February 2015

Authors:
Christopher Blair, C. Ryan Campbell, and Anne D. Yoder

Info:
Scientists at Duke University explored whether whole-genome amplification leads to sequence bias by performing a ddRAD-seq study of grey mouse lemurs using multiple displacement amplification. They conclude that MDA enrichment does not cause systematic bias, opening the doors for studies with low-quantity DNA. The team used Pippin Prep for size selection of the ddRAD-seq library.

Citation:
Molecular Ecology Resources (2015) 15, 1079–1090
doi: 10.1111/1755-0998.12376

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