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Category Archives: Blog
Cell Paper Reports Massive Genome Study for Lung Cancer
A paper that came out last month in Cell describes a massive effort to generate the exome and/or genome sequences of 183 tumor/normal pairs for people with a specific type of lung cancer. Authors hailed from Harvard University, New York … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep
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What’s in a Name?
We frequently get asked about the name “Pippin”, and why we’ve chosen it for our products. We thought it would be fun to see if the moniker has been also adapted in other fields of use — this is what … more »
Scientists Use Pippin in Sequencing Effort for Seven Cholera Isolates
A paper published this month in BMC Genomics discusses findings from a collaborative project to sequence cholera samples linked to the 2010 outbreak in Haiti — and we’re pleased to report that the Pippin platform from Sage Science was used … more »
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Tagged DNA Sequencing, Pippin Prep
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BluePippin “Mid-Range” Size Selection: 18-27 kb
We’re pleased to be shipping our Mid-Range size selection cassette (BMF7510) for the BluePippin. While our Low-Range cassette (BLF7510, 1kb-10kb) works for many mate-pair library construction protocols, we’ve been hearing from our customers that there is interest in creating larger … more »
Posted in Blog, Tips and Applications
Tagged BluePippin, Tips and Applications
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Publication: Spike-In Standards for Managing Systematic Sequencing Errors
Here’s a paper worth checking out: “Synthetic Spike-in Standards Improve Run-Specific Systematic Error Analysis for DNA and RNA Sequencing” from lead author Justin Zook at the National Institute of Standards and Technology. Published in PLoS One last month, the paper … more »
Posted in Blog
Tagged DNA Sequencing, Pippin Prep, Pippin System, RNA-seq
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For NRC Team, Pippin Platform Leads to Higher-Quality Assemblies
At the DNA Technologies Laboratory at the National Research Council of Canada, scientists are using the Pippin size selection platform to improve the quality of their genome assemblies. Andrew Sharpe, Research Officer and Group Leader of the Saskatoon-based laboratory, got … more »
Posted in Blog
Tagged BluePippin, DNA Sequencing, Illumina, Mate-pair Sequencing, Paired-end Sequencing, Pippin Prep, Pippin System
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BluePippin:Collecting all DNA “Greater Than” 1kb
We have received several requests to use the Pippin to collect all remaining DNA above a programmed base pair value from a sample. Although Pippins currently have the capability to collect all DNA after a run time threshold (using the … more »
Posted in Blog, Tips and Applications
Tagged BluePippin, Tips and Applications
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Broad Institute Teams with Sage Science for Automated Sizing
Don’t miss this great blog post from the Broad Institute (“A Sage partnership”) describing collaborative work between their genome sequencing team and Sage Science to design a better size selection process for the Broad’s sequencing pipeline. Headed up by Sheila … more »
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Tagged DNA Sequencing, Pippin Prep, Pippin System
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Automated Size Selection Key for RADseq: PLoS One Publication
Congratulations to Sage Science customer Hopi Hoekstra and her team at Harvard University for their recent publication in PLoS One! Dr. Hoekstra, who works in the Organismic & Evolutionary Biology and the Molecular & Cellular Biology departments at Harvard, reports … more »
Posted in Blog
Tagged Pippin Prep, RAD-seq
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At Emory, Size Selection Saves Time for NGS Prep
At the genome center at Emory University, scientists credit the Pippin Prep with shaving almost a full day off the sample prep process for Illumina’s mate-pair library prep. Chad Haase, laboratory manager at the Emory GRA Genome Center, says that … more »
Posted in Blog
Tagged DNA Sequencing, Illumina, Mate-pair Sequencing, Pippin Prep
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