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Category Archives: Blog
New Cassettes Use Internal Standards
We’ve just released new dye-free cassettes for the BluePippin and Pippin Prep that use dye-labeled DNA markers* as internal standards, instead of typical external marker set. In the new product, the labeled markers will come premixed wi th the Ficoll … more »
Posted in Blog, Tips and Applications
Tagged BluePippin, Pippin Prep, Pippin System, Tips and Applications
2 Comments
“Overflow Detection” definition on the Pippin Prep to be eliminated
| | Pippin Prep users are likely familiar with our “overflow detection” cassette definitions that are found in the “Cassette Type” drop down menu of the Protocol Editor. We use these definitions because Pippin cassettes exhibit a phenomenon where the … more »
Posted in Blog, Tips and Applications
Tagged Pippin Prep, Tips and Applications
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ChIP-Seq sample prep tip – Interpreting the apparent presence of a secondary band in size-selected DNA
ChIP-Seq analysis is used to study the control of gene expression by identifying DNA regions that are associated with chromatin at the time of cellular analysis. The ChIP-Seq procedure typically involves cross-linking DNA to the associated chromatin proteins, sonication to … more »
Posted in Blog, Tips and Applications
Tagged ChIP-Seq, DNA size selection, DNA sizing, NGS, NGS sample prep, Pippin Prep, Pippin System, Sage Science, Tips and Applications
Comments Off on ChIP-Seq sample prep tip – Interpreting the apparent presence of a secondary band in size-selected DNA