ASMS, Here We Come!
We are packing our bags for Minneapolis and the 61st annual conference of the American Society for Mass Spectrometry. As scientists who come from the DNA realm, the protein world is very exciting to us and we’re looking forward to one of the premier meetings in this field.
It’ll be great to see the award presentation from Dick Smith, head of proteomics research at Pacific Northwest National Laboratory. Dick has a long history of optimizing mass spec technology, so no doubt his talk will cover his greatest hits as well as what’s new and intriguing in his lab today. We’re also interested in hearing about cutting-edge work in top-down proteomics and other approaches during the regular conference sessions.
This is the first year Sage will have a booth at ASMS, so please stop by booth #31 and say hello! We’ll be introducing our two new automated tools for the protein market: the BluePippin for proteins and the Sage ELF. BluePippin, one of our DNA sizing platforms, has new cassettes to enable targeted SDS-protein collection of a desired fraction from up to five samples per run. The ELF, or Electrophoretic Lateral Fractionator, allows users to fractionate a protein sample into 12 contiguous size fractions. These tools provide increased reproducibility and ease-of-use for procedures that require gel isolation of proteins, including top-down and bottom-up proteomics studies, post-translational modification analysis, and other protein mass spectrometry methods.
You can also check out the two posters we’ll have, both during Wednesday’s poster session.
• Poster #590: “Using Orthogonal Techniques for Protein-Peptide Separation to Generate Comprehensive HDMSe Mass Spectral Libraries from an E. coli Model System”
• Poster #674: “The BluePippin Automated Size-Fractionation System for Proteins”
We hope to see you there!
New Prostate Cancer Research Indicates Rapid Accumulation of DNA Mutations
Researchers studying the behavior of prostate cancer have shed light on how the cancer develops mutations so rapidly. In contrast to other cancers that seem to progress by adding mutations one at a time, the prostate cancer samples that were analyzed showed evidence of a rapid chromosomal scramble resulting in several alterations that disrupt many genes at once. The scientists named this phenomenon “chromoplexy.”
The findings are described in “Punctuated Evolution of Prostate Cancer Genomes,” published in Cell by a large collaboration of scientists with senior authors Francesca Demichelis, Mark Rubin, and Levi Garraway. We were delighted to see that the team used Pippin Prep automated size selection as part of their sample preparation workflow in this impressive research.
For this work, the team sequenced 57 tumor genomes and their matched normal tissue, hoping to build on findings from earlier exome studies and to provide a better understanding of the onset and progression of prostate cancer. They also analyzed copy number changes across the genomes and studied the chromoplexy phenomenon they found, through which large chunks of the genomes had been jumbled in a short period of time. “These complex rearrangement events occur in the majority of prostate cancers and may commonly inactivate multiple tumor-constraining genes in a coordinated fashion,” the authors note.
They add, “Our modeling suggests that chromoplexy may induce considerable genomic derangement over relatively few events in prostate cancer and other neoplasms, supporting a model of punctuated cancer evolution.”
Pippin and PacBio: Better Together
Update: Just announced– Sage Science and Pacific Biosciences have partnered to offer users longer reads than ever. Click here to read the press release.
The new SMRTbellTM 20kb sample prep protocol using the BluePippin is posted on the PacBio® SampleNet site, click here to view the page or download the document. Instructions for using the BluePippin High Pass cassette definition for the SMRTbell protocol can be found here.
Original Post:
Many thanks to all the scientists who stopped by the Sage suite during the Advances in Genome Biology & Technology meeting in Marco Island — we were thrilled to see you! When we weren’t busy handing out glowing medallions, 
the Sage Science team had a great time catching up with people, attending the excellent scientific presentations, and walking the halls of the poster sessions.
Along with other attendees, we were really impressed by the posters and presentations showing data from the PacBio® RS. A number of talks referred to the company’s single-molecule, real-time (SMRT®) sequencing, which generates extremely long reads — one scientist said he’d produced a 26 kb read on the PacBio RS. Between the read length and unique base modification detection capability, the sequencing platform is particularly useful for de novo assembly, genome finishing, SNP discovery, and epigenetic analysis.
In its quest to produce sequence from even longer DNA fragments, PacBio recently tested our BluePippin platform for size selection; the results of those studies were the basis for a poster we displayed at AGBT. In it, the PacBio authors demonstrate a doubling of the N50 subread length – the value at which 50% of unique DNA bases are in reads longer than this value – when using the BluePippin platform compared to the standard workflow (click to enlarge this excerpt from the poster):
For generating 20 kb libraries that are optimal for the platform’s current polymerase, the authors note that “when combined with size selection to remove the shorter fragments that tend to load more favorably than long fragments, these libraries show greatly increased subread lengths.”
Looking at three examples of libraries for bacterial, fish, and mammalian DNA, the authors report that the N50 subread lengths increased by 90%, 110%, and 86%, respectively. Some of this data was also included in an AGBT plenary presentation by Jonas Korlach, Chief Scientific Officer of PacBio, who cited the BluePippin platform as one of the new improvements for PacBio customers.
Some PacBio customers are already using the BluePippin platform, and we look forward to working with more of them in the coming months. Together, these technologies offer a great and unique solution for truly long-read sequencing.
Pippin Pulse
Field Inversion Gel Electrophoresis
We’ve set up this post to provide Pippin Pulse users with a resource to keep updated on new protocols and to post references that might be useful. We also invite users to feel use this page as a forum, if you see fit, and we’d enjoy showing interesting gel images and data (email these to support@sagescience.com).
Our go-to book on theory:
“Electrophoresis of Large DNA Molecules:Theory and Applications” (E. Lai and B. Birren, eds), Current Communication in Cell & Molecular Biology Vol. 1,. Cold Spring Harbor Laboratory Press, New York, 1990
Download the Pippin Pulse user manual here
Our Gel Set-up
We use a Galileo Model 1214 RapidCastTM Mini Gel Unit running 12 X 14 cm gels using Lonza SeaKem® Gold Agarose. We use our Pippin Tris-TAPS buffers (which you can order directly from us in the US and Canada, Part No. KBB1001, under “Accessories” on our ordering page) or 0.5X TBE. The formulations can be found in the Pippin Pulse user manual or separately here.
Gel images of our 1-50kb and 3-70kb protocols:
(click to enlarge images)
Gel images of our 5-430kb and 5-150kb protocols:
Improved Recovery for 3-10kb DNA Size Selection
BluePippin users, we have a new cassette definition that will appear in the Cassette Definition Set 10 (CD10) that we feel merits some further detail. This definition is named “0.75% DF 3 – 10kb Marker S1 – Improved Recovery”
What is it?
This definition improves the sample recovery for customers using BLF7510 Marker S1 cassette kits for DNA size selection, for size ranges between 3- 10 kb. The original cassette definition for this range, “0.75% DF 3 – 10kb Marker S1” uses pulsed-field during elution (size selection), and we have subsequently found that direct-current elution improves recovery.
Who should use it?
This is now the recommended cassette definition to use for size-selecting between 3-10kb. However, if you have optimized a protocol using the current version you may want continue to use it (it will not be deleted as a cassette definition option) if you do not wish to re-optimize.
Also note that since the BluePippin system does not allow for simultaneous pulsed-field and direct-current operation, lanes that are being separated using pulsed-field will temporarily be halted during every elution process. This makes the total run times lengths dependent on the elution times (and size selection targets), and therefore more difficult to estimate and can add up to 40 minutes to a cassette run.
How much improved is the recovery?
Our validation of the change in the cassette definition shows up to a 2-fold increase in recovery. As you may know, for collections of large fragments (on 0.75% agarose cassettes) we provide Tween solution which we find improves recovery if one employs a post-collection Tween rinse. Considered together, here are some results from our study. We started with a 5 ug of restriction digested DNA, and 8kb “Tight” collections:
