Comprehensive Transcriptome Profiling of Balding and Non-Balding Scalps in Trichorhinophalangeal Syndrome Type I Patient
October 2017
Authors:
Yun-Ji Kim, Byulee Yoon, Kyudong Han, Byung Cheol Park
Info:
Scientists in Korea compared transcriptomes of RNA extracted from balding and non-balding scalp regions on the same patient to understand the genetics of hair loss. They found more than 1,200 differentially expressed genes between the samples. Sequencing was performed on an Illumina HiSeq 2500, with size selection conducted on BluePippin.
Citation:
Ann Dermatol. 2017 Oct;29(5):597-601
doi: 10.5021/ad.2017.29.5.597
Micro RNA’s (miRNA’s) may help explain expression of multiple genes in Alzheimer’s Frontal Cortex
September 2017
Authors:
James Bennett and Paula Keeney
Info:
Scientists from Neurodegeneration Therapeutics used RNA-seq to compare microRNAs found in Alzheimer’s brain samples and controls. They identified 55 up-regulated genes and 191 down-regulated genes in the Alzheimer’s samples. PippinHT was used for small RNA size selection ahead of sequencing on an Illumina NextSeq500.
Citation:
Journal of Systems and Integrative Neuroscience
http://www.oatext.com/micro-rnas-mirnas-may-help-explain-expression-of-multiple-genes-in-alzheimers-frontal-cortex.php#Article
Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method
September 2017
Authors:
Kengo Kato, Masanori Hashino, Tamaki Ito, Mari Matsui, et al.
Info:
Researchers developed and validated a streamlined protocol for PacBio sequencing sample prep. The method involves bead-beating for DNA isolation. For the project, the team used Pippin Pulse to perform automated electrophoresis.
Citation:
J Biol Methods 2017;4(3):e79.
doi: 10.14440/jbm.2017.169
Single molecule counting and assessment of random molecular tagging errors with transposable giga-scale error-correcting barcodes
September 2017
Authors:
Billy Lau and Hanlee Ji
Info:
Scientists aimed to address errors that occur with conventional molecular barcodes for RNA-seq workflows. They developed transposable error-correcting barcodes with tens of billions of unique labels and found that this approach mitigates such problems. They validated the approach in a project that incorporated Pippin Prep to remove PCR products smaller than 500 bp.
Citation:
BMC Genomics 201718:745
doi: 10.1186/s12864-017-4141-4