Citations

Pitfalls of haplotype phasing from amplicon-based long-read sequencing

February 2016

Authors:
Thomas W. Laver, Richard C. Caswell, Karen A. Moore, Jeremie Poschmann, Matthew B. Johnson, Martina M. Owens, Sian Ellard, Konrad H. Paszkiewicz & Michael N. Weedon

Info:
University of Exeter researchers used long-range PCR and long-read sequencing to phase genetic variants separated by 9 Kb, comparing results from the PacBio and Oxford Nanopore sequencing systems. BluePippin was used for size selection of the PacBio 20 Kb library.

Citation:
Scientific Reports 6, Article number: 21746 (2016)
doi:10.1038/srep21746

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Comparison of Sample Preparation Methods Used for the Next-Generation Sequencing of Mycobacterium tuberculosis

February 2016

Authors:
Andrea D. Tyler, Sara Christianson, Natalie C. Knox, Philip Mabon, Joyce Wolfe, Gary Van Domselaar, Morag R. Graham, and Meenu K. Sharma

Info:
In this PLoS One publication, scientists in Manitoba compare the quality of libraries constructed with Illumina’s Nextera and TruSeq protocols for Mycobacterium tuberculosis, which has a particularly challenging genome. They determined that the TruSeq method yielded more uniform data quality and coverage. BluePippin was used for size selection prior to sequencing on a MiSeq.

Citation:

DOI: 10.1371/journal.pone.0148676

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The Many Dimensions of Diet Breadth: Phytochemical, Genetic, Behavioral, and Physiological Perspectives on the Interaction between a Native Herbivore and an Exotic Host

February 2016

Authors:
Joshua G. Harrison, Zachariah Gompert, James A. Fordyce, C. Alex Buerkle, Rachel Grinstead, Joshua P. Jahner, Scott Mikel, Christopher C. Nice, Aldrin Santamaria, and Matthew L. Forister

Info:
In this study, scientists analyzed butterflies colonizing several different populations of alfalfa, finding significant variation in larval performance between plant groups. Plant DNA was sequenced on an Illumina HiSeq after size selection of library fragments with BluePippin.

Citation:
PLoS One
DOI: 10.1371/journal.pone.0147971

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Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures

January 2016

Authors:
Lowiese M. B. Desmarets, Ben L. Vermeulen, Sebastiaan Theuns, Nádia Conceição-Neto, Mark Zeller, Inge D. M. Roukaerts, Delphine D. Acar, Dominique A. J. Olyslaegers, Marc Van Ranst, Jelle Matthijnssens, and Hans J. Nauwyncka

Info:
Scientists in Belgium studied fecal samples from three cats to understand evolutionary mutations in the feline enteric coronavirus genome, which can cause feline infectious peritonitis. DNA libraries were size-selected with BluePippin prior to sequencing on an Illumina HiSeq.

Citation:
Sci Rep. 2016; 6: 20022

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Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress

January 2016

Authors:
B. Shaun Bushman, Keenan L. Amundsen, Scott E. Warnke, Joseph G. Robins, and Paul G. Johnson

Info:
Scientists compared the transcriptomes of two strains of Kentucky bluegrass, one sensitive to salt and the other tolerant even to frequent exposure to salt. cDNA sequencing was performed on an Ion Torrent Proton after library size selection on the BluePippin.

Citation:
BMC Genomics

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