Patterns of genome-wide variation in Glossina fuscipes fuscipes tsetse flies from Uganda
April 2016
Authors:
Gloria-Soria A, Dunn WA, Telleria EL, Evans BR, Okedi L, Echodu R, Warren WC, Montague MJ, Aksoy S, Caccone A
Info:
Researchers used ddRAD-seq to develop a panel of SNPs for use in population genomics or genome-wide association studies for the tsetse fly Glossina fuscipes fuscipes, a vector for Human African Trypanosomiasis. They performed size selection with BluePippin prior to sequencing.
Citation:
G3: Genes|Genomes|Genetics
doi:10.1534/g3.116.027235
Long-read sequence assembly of the gorilla genome
April 2016
Authors:
David Gordon, John Huddleston, Mark J. P. Chaisson, Christopher M. Hill, Zev N. Kronenberg, et al.
Info:
Scientists from the University of Washington and other institutions report a new gorilla genome assembly, which improves on the quality of previous assemblies by 150-fold by incorporating long-read sequencing. The team used BluePippin to remove smaller library fragments prior to sequencing on the PacBio system.
Citation:
Science 01 Apr 2016: Vol. 352, Issue 6281
DOI: 10.1126/science.aae0344
Multiplexed Sequence Encoding: A Framework for DNA Communication
April 2016
Authors:
Bijan Zakeri, Peter A. Carr, and Timothy K. Lu
Info:
MIT scientists present new methods to use synthetic DNA for communicating messages, including encoding and encrypting messages over several DNA fragments. BluePippin was used in sequencing the synthetic DNA.
Citation:
PLoS One
http://dx.doi.org/10.1371/journal.pone.0152774
Design and synthesis of a minimal bacterial genome
March 2016
Authors:
Clyde A. Hutchison III, Ray-Yuan Chuang, Vladimir N. Noskov, et al.
Info:
Scientists from the J. Craig Venter Institute and other institutes systematically stripped out genes from the Mycoplasma mycoides genome until they were down to the 473 genes necessary for the organism’s survival. They used BluePippin with the PacBio RS II for de novo assembly of the artificial genome.
Citation:
Science 25 Mar 2016: Vol. 351, Issue 6280
DOI: 10.1126/science.aad6253
Hybridization Capture Using RAD Probes (hyRAD), a New Tool for Performing Genomic Analyses on Collection Specimens
March 2016
Authors:
Tomasz Suchan, Camille Pitteloud, Nadezhda S. Gerasimova, Anna Kostikova, Sarah Schmid, Nils Arrigo, Mila Pajkovic, Michał Ronikier, and Nadir Alvarez
Info:
Scientists present a new protocol based on the RAD-seq concept that works more effectively for samples with degraded DNA, such as museum samples. They demonstrate the approach in butterflies and grasshoppers. Pippin Prep was used for size selection of ligation products prior to sequencing.
Citation:
PLoS ONE 11(3): e0151651.
doi:10.1371/journal.pone.0151651