The Many Dimensions of Diet Breadth: Phytochemical, Genetic, Behavioral, and Physiological Perspectives on the Interaction between a Native Herbivore and an Exotic Host
February 2016
Authors:
Joshua G. Harrison, Zachariah Gompert, James A. Fordyce, C. Alex Buerkle, Rachel Grinstead, Joshua P. Jahner, Scott Mikel, Christopher C. Nice, Aldrin Santamaria, and Matthew L. Forister
Info:
In this study, scientists analyzed butterflies colonizing several different populations of alfalfa, finding significant variation in larval performance between plant groups. Plant DNA was sequenced on an Illumina HiSeq after size selection of library fragments with BluePippin.
Citation:
PLoS One
DOI: 10.1371/journal.pone.0147971
Experimental feline enteric coronavirus infection reveals an aberrant infection pattern and shedding of mutants with impaired infectivity in enterocyte cultures
January 2016
Authors:
Lowiese M. B. Desmarets, Ben L. Vermeulen, Sebastiaan Theuns, Nádia Conceição-Neto, Mark Zeller, Inge D. M. Roukaerts, Delphine D. Acar, Dominique A. J. Olyslaegers, Marc Van Ranst, Jelle Matthijnssens, and Hans J. Nauwyncka
Info:
Scientists in Belgium studied fecal samples from three cats to understand evolutionary mutations in the feline enteric coronavirus genome, which can cause feline infectious peritonitis. DNA libraries were size-selected with BluePippin prior to sequencing on an Illumina HiSeq.
Citation:
Sci Rep. 2016; 6: 20022
Transcriptome profiling of Kentucky bluegrass (Poa pratensis L.) accessions in response to salt stress
January 2016
Authors:
B. Shaun Bushman, Keenan L. Amundsen, Scott E. Warnke, Joseph G. Robins, and Paul G. Johnson
Info:
Scientists compared the transcriptomes of two strains of Kentucky bluegrass, one sensitive to salt and the other tolerant even to frequent exposure to salt. cDNA sequencing was performed on an Ion Torrent Proton after library size selection on the BluePippin.
Citation:
BMC Genomics
A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling
January 2016
Authors:
Rosalinda D’Amore, Umer Zeeshan Ijaz, Melanie Schirmer, John G. Kenny, Richard Gregory, Alistair C. Darby, Migun Shakya, Mircea Podar, Christopher Quince and Neil Hall
Info:
Scientists used synthetic metagenomic communities to evaluate the performance of different sequencing and analysis pipelines. They assessed platforms including MiSeq, the PacBio RS II, 454 GS-FLX, and Ion Torrent, and found that sample preparation can alter data interpretation. They used Pippin Prep for sizing NGS libraries prior to sequencing.
Citation:
D’Amore et al. BMC Genomics (2016) 17:55
DOI 10.1186/s12864-015-2194-9
Harnessing the power of RADseq for ecological and evolutionary genomics
January 2016
Authors:
Kimberly R. Andrews, Jeffrey M. Good, Michael R. Miller, Gordon Luikart and Paul A. Hohenlohe
Info:
In this protocol review, scientists examine several approaches to restriction site-associated DNA sequencing and present their findings on how to choose the best method for a specific project. Pippin is included as a key step in the original RADseq protocol.
Citation:
Nature Reviews Genetics
doi:10.1038/nrg.2015.28