The mammalian LINC complex regulates genome transcriptional responses to substrate rigidity
December 2016
Authors:
Samer G. Alam, Qiao Zhang, Nripesh Prasad, Yuan Li, Srikar Chamala, et al.
Info:
Researchers from the HudsonAlpha Institute, the University of Florida, and other institutions studied the role of rigidity in the extracellular matrix, finding that the regulation of certain genes relies on nuclear tension. They note that this is the first report of how the linker of nucleoskeleton and cytoskeleton complex alters gene expression throughout the genome. Pippin Prep was used to size-select small RNAs for building a microRNA library.
Citation:
Scientific Reports 6, Article number: 38063 (2016)
doi:10.1038/srep38063
Selective Y centromere inactivation triggers chromosome shattering in micronuclei and repair by non-homologous end joining
December 2016
Authors:
Peter Ly, Levi S. Teitz, Dong H. Kim, Ofer Shoshani, Helen Skaletsky, Daniele Fachinetti, David C. Page, and Don W. Cleveland
Info:
Scientists report developing a strategy to examine what happens when chromosomes do not segregate properly. They found that missegregation into a micronucleus leads to a cascade of events affecting several cell cycles. PippinHT was used for size selection prior to paired-end sequencing on an Illumina HiSeq 2500 instrument.
Citation:
Nature Cell Biology 19, 68–75 (2017)
doi:10.1038/ncb3450
Susceptibility to Aminoglycosides and Distribution of aph and aac(3)-XI Genes among Corynebacterium striatum Clinical Isolates
December 2016
Authors:
Jesús Navas, Marta Fernández-Martínez, Carlos Salas, María Eliecer Cano, Luis Martínez-Martínez
Info:
Scientists in Spain analyzed several strains of the pathogen Corynebacterium striatum and found that many were resistant to drugs most commonly used to treat these infections. They used BluePippin to size-select libraries prior to sequencing on an Illumina MiSeq.
Citation:
PLoS One 11(12): e0167856
doi:10.1371/journal.pone.0167856
Single-Molecule Sequencing (PacBio) of the Staphylococcuscapitis NRCS-A Clone Reveals the Basis of Multidrug Resistance and Adaptation to the Neonatal Intensive Care Unit Environment
December 2016
Authors:
Patrícia MartinsSimões, HajarLemriss, YannDumont, SanâaLemriss, et al.
Info:
Scientists in France and Morocco fully sequenced the genome and accessory genome of a Staphylococcus capitis clone that has proven resistant to many drugs and is particularly detrimental to newborns. They identified many elements of antimicrobial resistance, including six genes carried on mobile genetic elements. BluePippin was used for size selection prior to sequencing on the PacBio RS II instrument.
Citation:
Frontiers in Microbiology
Generation of Full-Length Class I Human Leukocyte Antigen Gene Consensus Sequences for Novel Allele Characterization
December 2016
Authors:
Peter M. Clark, Jamie L. Duke, Deborah Ferriola, Valia Bravo-Egana, Tunde Vago, Aniqa Hassan, Anna Papazoglou, and Dimitri Monos
Info:
Scientists from The Children’s Hospital of Philadelphia evaluated an NGS-based HLA typing kit from Omixon. They analyzed 50 samples; 7.7% were found to have novel polymorphisms, suggesting that new sequencing approaches could usher in a new era of discovery for HLA alleles. The team used Pippin Prep to size-select libraries prior to sequencing on a MiSeq.
Citation:
Clinical Chemistry
DOI: 10.1373/clinchem.2016.260661