Small RNA populations revealed by blocking rRNA fragments in Drosophila melanogaster reproductive tissues
February 2018
Authors:
Emily Fowler, Irina Mohorianu, Damian Smith, Tamas Dalmay, Tracey Chapman
Info:
Scientists present a method for removing highly abundant rRNAs for improved analysis of small RNAs in RNA interference studies. Results showed increased resolution of sRNAs, even those seen at low frequency. For this work, the team cited BluePippin as a commonly used size selection tool for preparing sRNA-seq libraries.
Citation:
PLoS One
doi: 10.1371/journal.pone.0191966
The DNA Methylome of the Hyperthermoacidophilic Crenarchaeon Sulfolobus acidocaldarius
February 2018
Authors:
Mohea Couturier and Ann-Christin Lindås
Info:
Scientists from Stockholm University investigated the impact of DNA methylation in archaeal genomes by analyzing the complete DNA methylome of S. acidocaldarius using PacBio sequencing. The team used BluePippin to remove short fragments prior to sequencing.
Citation:
Front. Microbiol.
doi: 10.3389/fmicb.2018.00137
Draft genome assembly of the Bengalese finch, Lonchura striata domestica, a model for motor skill variability and learning
February 2018
Authors:
Bradley Colquitt, David Mets, and Michael Brainard
Info:
Scientists generated a high-quality draft genome and gene annotation for Bengalese finch, which has a more variable song than other finches, making it an important model for sensorimotor learning. They used BluePippin for size selection prior to sequencing.
Citation:
GigaScience
doi: 10.1093/gigascience/giy008
Ribonuclease Inhibitor 1 regulates erythropoiesis by controlling GATA1 translation
February 2018
Authors:
Vijaykumar Chennupati, Diogo Veiga, Kendle Maslowski, Nicola Andina, et al.
Info:
Scientists studying the ribonuclease inhibitor protein found that it controls translation of GATA1 and thereby regulates erythropoiesis, helping to explain anemia-associated diseases caused by mutations in ribosomal proteins. For this work, the team size-selected libraries for an RNA-seq workflow using a PippinHT instrument.
Citation:
J Clin Invest.
doi:10.1172/JCI94956
Oligomeric proanthocyanidins (OPCs) target cancer stem-like cells and suppress tumor organoid formation in colorectal cancer
February 2018
Authors:
Shusuke Toden, Preethi Ravindranathan, Jinghua Gu, Jacob Cardenas, Madelaine Yuchang &
Ajay Goel
Info:
Scientists from Baylor University Medical Center studied the chemoprotective role of oligomeric proanthocyanidins, finding that they inhibited cellular proliferation and spurred apoptosis. RNA sequencing data showed that these elements disrupted self-renewal pathways. The team used PippinHT to size-select pooled libraries prior to RNA sequencing.
Citation:
Scientific Reports
Vol 8, Article number: 3335(2018)
doi:10.1038/s41598-018-21478-8